2W35

Structures of endonuclease V with DNA reveal initiation of deaminated adenine repair

2W35 の概要

• エントリーDOI: 10.2210/pdb2w35/pdb
• 関連するPDBエントリー: 2W36
• 分子名称: Endonuclease V, 5'-D(*AP*TP*GP*CP*GP*AP*CP*IP*GP)-3', 5'-D(*AP*GP*CP*CP*GP*TP)-3', ... (5 entities in total)
• 機能のキーワード: hypoxanthine, endonuclease, endonucleasev, hydrolase, inosine, dna damage, dna repair
• 由来する生物種: Thermotoga maritima; 詳細
• 細胞内の位置: Cytoplasm (By similarity): Q9X2H9
• タンパク質・核酸の鎖数: 6
• 化学式量合計: 60470.57

構造登録者

Dalhus, B.,Arvai, A.S.,Rosnes, I.,Olsen, O.E.,Backe, P.H.,Alseth, I.,Gao, H.,Cao, W.,Tainer, J.A.,Bjoras, M. (登録日: 2008-11-06, 公開日: 2009-01-20, 最終更新日: 2024-06-19)

主引用文献

Dalhus, B.,Arvai, A.S.,Rosnes, I.,Olsen, O.E.,Backe, P.H.,Alseth, I.,Gao, H.,Cao, W.,Tainer, J.A.,Bjoras, M.
Structures of Endonuclease V with DNA Reveal Initiation of Deaminated Adenine Repair.
Nat.Struct.Mol.Biol., 16:138-, 2009

PubMed Abstract: Endonuclease V (EndoV) initiates a major base-repair pathway for nitrosative deamination resulting from endogenous processes and increased by oxidative stress from mitochondrial dysfunction or inflammatory responses. We solved the crystal structures of Thermotoga maritima EndoV in complex with a hypoxanthine lesion substrate and with product DNA. The PYIP wedge motif acts as a minor groove-damage sensor for helical distortions and base mismatches and separates DNA strands at the lesion. EndoV incises DNA with an unusual offset nick 1 nucleotide 3' of the lesion, as the deaminated adenine is rotated approximately 90 degrees into a recognition pocket approximately 8 A from the catalytic site. Tight binding by the lesion-recognition pocket in addition to Mg(2+) and hydrogen-bonding interactions to the DNA ends stabilize the product complex, suggesting an orderly recruitment of downstream proteins in this base-repair pathway.

PubMed: 19136958
DOI: 10.1038/NSMB.1538

実験手法

X-RAY DIFFRACTION (2.15 Å)