2W2T

Rac2 (G12V) in complex with GDP

Summary for 2W2T

• Entry DOI: 10.2210/pdb2w2t/pdb
• Related: 1DS6
• Descriptor: RAS-RELATED C3 BOTULINUM TOXIN SUBSTRATE 2, MAGNESIUM ION, GUANOSINE-5'-DIPHOSPHATE, ... (4 entities in total)
• Functional Keywords: signaling protein, phospholipase c, phosphoinositides, rho gtpases, rac signaling protein
• Biological source: HOMO SAPIENS (HUMAN)
• Total number of polymer chains: 1
• Total formula weight: 20777.80

Authors

Opaleye, O.,Bunney, T.D.,Roe, S.M.,Pearl, L.H. (deposition date: 2008-11-04, release date: 2009-05-05, Last modification date: 2024-05-01)

Primary citation

Bunney, T.D.,Opaleye, O.,Roe, S.M.,Vatter, P.,Baxendale, R.W.,Walliser, C.,Everett, K.L.,Josephs, M.B.,Christow, C.,Rodrigues-Lima, F.,Gierschik, P.,Pearl, L.H.,Katan, M.
Structural Insights Into Formation of an Active Signaling Complex between Rac and Phospholipase C Gamma 2.
Mol.Cell, 34:223-, 2009

PubMed Abstract: Rho family GTPases are important cellular switches and control a number of physiological functions. Understanding the molecular basis of interaction of these GTPases with their effectors is crucial in understanding their functions in the cell. Here we present the crystal structure of the complex of Rac2 bound to the split pleckstrin homology (spPH) domain of phospholipase C-gamma(2) (PLCgamma(2)). Based on this structure, we illustrate distinct requirements for PLCgamma(2) activation by Rac and EGF and generate Rac effector mutants that specifically block activation of PLCgamma(2), but not the related PLCbeta(2) isoform. Furthermore, in addition to the complex, we report the crystal structures of free spPH and Rac2 bound to GDP and GTPgammaS. These structures illustrate a mechanism of conformational switches that accompany formation of signaling active complexes and highlight the role of effector binding as a common feature of Rac and Cdc42 interactions with a variety of effectors.

PubMed: 19394299
DOI: 10.1016/J.MOLCEL.2009.02.023

Experimental method

X-RAY DIFFRACTION (1.95 Å)