2W00

Crystal structure of the HsdR subunit of the EcoR124I restriction enzyme in complex with ATP

2W00 の概要

• エントリーDOI: 10.2210/pdb2w00/pdb
• 分子名称: HSDR, ADENOSINE-5'-TRIPHOSPHATE, MAGNESIUM ION, ... (4 entities in total)
• 機能のキーワード: atp-binding, dna-binding, restriction system, helicase, hydrolase, r.ecor124i, nucleotide-binding, type i restriction-modification enzyme
• 由来する生物種: ESCHERICHIA COLI
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 243027.63

構造登録者

Lapkouski, M.,Panjikar, S.,Kuta Smatanova, I.,Ettrich, R.,Csefalvay, E. (登録日: 2008-08-08, 公開日: 2008-12-16, 最終更新日: 2024-10-16)

主引用文献

Lapkouski, M.,Panjikar, S.,Janscak, P.,Smatanova, I.K.,Carey, J.,Ettrich, R.,Csefalvay, E.
Structure of the Motor Subunit of Type I Restriction-Modification Complex Ecor124I.
Nat.Struct.Mol.Biol., 16:94-, 2009

PubMed Abstract: Type I restriction-modification enzymes act as conventional adenine methylases on hemimethylated DNAs, but unmethylated recognition targets induce them to translocate thousands of base pairs before cleaving distant sites nonspecifically. The first crystal structure of a type I motor subunit responsible for translocation and cleavage suggests how the pentameric translocating complex is assembled and provides a structural framework for translocation of duplex DNA by RecA-like ATPase motors.

PubMed: 19079266
DOI: 10.1038/NSMB.1523

実験手法

X-RAY DIFFRACTION (2.6 Å)