2VZY

Crystal structure of Rv0802c from Mycobacterium tuberculosis in an unliganded form.

2VZY の概要

• エントリーDOI: 10.2210/pdb2vzy/pdb
• 関連するPDBエントリー: 2VZZ
• 分子名称: RV0802C, ACETATE ION, SULFATE ION, ... (7 entities in total)
• 機能のキーワード: transferase, gcn5-related n-acetyltransferase, mycobacterium tuberculosis, rv0802c, succinyltransferase
• 由来する生物種: MYCOBACTERIUM TUBERCULOSIS
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 101660.72

構造登録者

Vetting, M.W.,Errey, J.C.,Blanchard, J.S. (登録日: 2008-08-07, 公開日: 2009-04-07, 最終更新日: 2024-05-08)

主引用文献

Vetting, M.W.,Errey, J.C.,Blanchard, J.S.
Rv0802C from Mycobacterium Tuberculosis: The First Structure of a Succinyltransferase with the Gnat Fold.
Acta Crystallogr.,Sect.F, 64:978-, 2008

PubMed Abstract: Gene rv0802c from Mycobacterium tuberculosis encodes a 218-amino-acid protein and is annotated as a hypothetical protein with homology to GCN5-related N-acetyltransferases. The structure of Rv0802c was determined in an unliganded form to 2.0 A resolution utilizing single-wavelength anomalous dispersion from a samarium soak that resulted in a single bound Sm(3+):citrate(2) complex. The structure confirms that Rv0802c exhibits the GCN5-related N-acetyltransferase fold and revealed a tetramer composed of a dimer of dimers with approximate 222 symmetry. In addition, a bound acetate ion indicated that Rv0802c may utilize a unique acyl donor for the family. The subsequent determination of the structure of Rv0802c in complex with succinyl-CoA to 2.3 A resolution suggests that Rv0802c is the first known GCN5-related N-acetyltransferase family member to utilize succinyl-CoA as a substrate.

PubMed: 18997321
DOI: 10.1107/S1744309108031679

実験手法

X-RAY DIFFRACTION (2 Å)