2VYT

The MBT repeats of human SCML2 bind to peptides containing mono methylated lysine.

2VYT の概要

• エントリーDOI: 10.2210/pdb2vyt/pdb
• 関連するPDBエントリー: 1OI1 2BIV
• 分子名称: SEX COMB ON MIDLEG-LIKE PROTEIN 2, N-METHYL-LYSINE, TRIETHYLENE GLYCOL, ... (4 entities in total)
• 機能のキーワード: mono methylated peptides, mbt repeats, transcription, phosphoprotein, alternative splicing, transcription regulation, nucleus, repressor, chromatin, human scml2
• 由来する生物種: HOMO SAPIENS (HUMAN)
• 細胞内の位置: Nucleus : Q9UQR0
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 49772.24

構造登録者

Santiveri, C.M.,Lechtenberg, B.C.,Allen, M.D.,Sathyamurthy, A.,Jaulent, A.M.,Freund, S.M.V.,Bycroft, M. (登録日: 2008-07-28, 公開日: 2008-08-05, 最終更新日: 2023-12-13)

主引用文献

Santiveri, C.M.,Lechtenberg, B.C.,Allen, M.D.,Sathyamurthy, A.,Jaulent, A.M.,Freund, S.M.V.,Bycroft, M.
The Malignant Brain Tumor Repeats of Human Scml2 Bind to Peptides Containing Monomethylated Lysine.
J.Mol.Biol., 382:1107-, 2008

PubMed Abstract: SCML2 (sex comb on midleg-like 2) is a constituent of the Polycomb repressive complex 1, a large multiprotein assembly required for the repression of developmental control genes. It contains two MBT (malignant brain tumor) repeats; the MBT is a protein module structurally similar to domains that bind to methylated histones. We have used NMR spectroscopy to examine the binding specificity of these repeats. Our data show that they preferentially bind histone peptides monomethylated at lysine residues with no apparent sequence specificity. The crystal structure of the complex between the protein and monomethyllysine reveals that the modified amino acid binds to an aromatic rich pocket at one end of the beta-barrel of the second repeat.

PubMed: 18706910
DOI: 10.1016/J.JMB.2008.07.081

実験手法

X-RAY DIFFRACTION (1.9 Å)