2VY5

U11-48K CHHC Zn-finger protein domain

2VY5 の概要

• エントリーDOI: 10.2210/pdb2vy5/pdb
• 関連するPDBエントリー: 2VY4
• 分子名称: U11/U12 SMALL NUCLEAR RIBONUCLEOPROTEIN 48 KDA PROTEIN, ZINC ION (2 entities in total)
• 機能のキーワード: splicing, mrna processing, alternative splicing, transcription, nucleus, spliceosome, polymorphism, mrna splicing
• 由来する生物種: HOMO SAPIENS (HUMAN)
• 細胞内の位置: Nucleus : Q6IEG0
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 4253.30

構造登録者

Tidow, H.,Andreeva, A.,Rutherford, T.J.,Fersht, A.R. (登録日: 2008-07-18, 公開日: 2009-02-17, 最終更新日: 2024-05-15)

主引用文献

Tidow, H.,Andreeva, A.,Rutherford, T.J.,Fersht, A.R.
Solution structure of the U11-48K CHHC zinc-finger domain that specifically binds the 5' splice site of U12-type introns.
Structure, 17:294-302, 2009

PubMed Abstract: The formation of stable 18S U11/U12 di-snRNPs before their association with the pre-mRNA is a characteristic feature of the minor spliceosome. During the spliceosomal assembly, the 18S snRNP binds cooperatively to the introns' 5' splice and branch point site. The molecular basis for this recognition is still unknown. Here, we report the solution structure of the U11-48K CHHC Zn finger, a domain unique to the minor spliceosome. The CHHC Zn-finger structure revealed an unexpected similarity to the TFIIIA domains, with distinct features originating from the type and separation of the zinc-coordinating residues. We show that this domain specifically binds the 5' splice site sequence of U12-type introns when base paired to U11 snRNA in vitro and hence may contribute to the U12 intron recognition. We propose a model in which the U11-48K Zn finger stabilizes U11-5' splice site base pairing and thus plays an important role during the minor spliceosome assembly.

PubMed: 19217400
DOI: 10.1016/j.str.2008.11.013

実験手法

SOLUTION NMR