2VWK

Uracil Recognition in Archaeal DNA Polymerases Captured by X-ray Crystallography. V93Q polymerase variant

2VWK の概要

• エントリーDOI: 10.2210/pdb2vwk/pdb
• 関連するPDBエントリー: 1TGO 1WN7 2VWJ
• 分子名称: DNA POLYMERASE, SODIUM ION, SULFATE ION, ... (4 entities in total)
• 機能のキーワード: dna replication, multifunctional enzyme, nucleotidyltransferase, dna-directed dna polymerase, transferase, exonuclease, dna-binding, dna polymerase, uracil, archaea, nuclease, hydrolase, dna repair
• 由来する生物種: THERMOCOCCUS GORGONARIUS
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 90660.74

構造登録者

Firbank, S.J.,Wardle, J.,Heslop, P.,Lewis, R.J.,Connolly, B.A. (登録日: 2008-06-26, 公開日: 2008-07-22, 最終更新日: 2024-10-23)

主引用文献

Firbank, S.J.,Wardle, J.,Heslop, P.,Lewis, R.J.,Connolly, B.A.
Uracil Recognition in Archaeal DNA Polymerases Captured by X-Ray Crystallography.
J.Mol.Biol., 381:529-, 2008

PubMed Abstract: Archaeal family B DNA polymerases bind tightly to template-strand uracil and stall replication on encountering the pro-mutagenic base. This article describes an X-ray crystal structure, at 2.8 A resolution, of Thermococcus gorgonarius polymerase in complex with a DNA primer-template containing uracil in the single-stranded region. The DNA backbone is distorted to position the uracil deeply within a pocket, located in the amino-terminal domain of the polymerase. Specificity arises from a combination of hydrogen bonds between the protein backbone and uracil, with the pocket shaped to prevent the stable binding of the four standard DNA bases. Strong interactions are seen with the two phosphates that flank the uracil and the structure gives clues concerning the coupling of uracil binding to the halting of replication. The importance of key amino acids, identified by the analysis of the structure and their conservation between archaeal polymerases, was confirmed by site-directed mutagenesis. The crystal structure of V93Q, a polymerase variant that no longer recognises uracil, is also reported, explaining the V93Q phenotype by the steric exclusion of uracil from the pocket.

PubMed: 18614176
DOI: 10.1016/J.JMB.2008.06.004

実験手法

X-RAY DIFFRACTION (2.6 Å)