2VSI

Synthesis of CDP-activated ribitol for teichoic acid precursors in Streptococcus pneumoniae

2VSI の概要

• エントリーDOI: 10.2210/pdb2vsi/pdb
• 関連するPDBエントリー: 2VSH
• 分子名称: 2-C-METHYL-D-ERYTHRITOL 4-PHOSPHATE CYTIDYLYLTRANSFERASE, 4-AMINO-1-{5-O-[(R)-HYDROXY(PHOSPHONOOXY)PHOSPHORYL]-ALPHA-D-ARABINOFURANOSYL}PYRIMIDIN-2(1H)-ONE, CALCIUM ION, ... (4 entities in total)
• 機能のキーワード: spr1149 tari cdp ribitol streptococcus pneumoniae, nucleotidyltransferase, transferase, isoprene biosynthesis
• 由来する生物種: STREPTOCOCCUS PNEUMONIAE
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 53671.01

構造登録者

Baur, S.,Marles-Wright, J.,Buckenmaier, S.,Lewis, R.J.,Vollmer, W. (登録日: 2008-04-23, 公開日: 2008-12-30, 最終更新日: 2023-12-13)

主引用文献

Baur, S.,Marles-Wright, J.,Buckenmaier, S.,Lewis, R.J.,Vollmer, W.
Synthesis of Cdp-Activated Ribitol for Teichoic Acid Precursors in Streptococcus Pneumoniae.
J.Bacteriol., 191:1200-, 2009

PubMed Abstract: Streptococcus pneumoniae has unusually complex cell wall teichoic acid and lipoteichoic acid, both of which contain a ribitol phosphate moiety. The lic region of the pneumococcal genome contains genes for the uptake and activation of choline, the attachment of phosphorylcholine to teichoic acid precursors, and the transport of these precursors across the cytoplasmic membrane. The role of two other, so far uncharacterized, genes, spr1148 and spr1149, in the lic region was determined. TarJ (spr1148) encodes an NADPH-dependent alcohol dehydrogenase for the synthesis of ribitol 5-phosphate from ribulose 5-phosphate. TarI (spr1149) encodes a cytidylyl transferase for the synthesis of cytidine 5'-diphosphate (CDP)-ribitol from ribitol 5-phosphate and cytidine 5'-triphosphate. We also present the crystal structure of TarI with and without bound CDP, and the structures present a rationale for the substrate specificity of this key enzyme. No transformants were obtained with insertion plasmids designed to interrupt the tarIJ genes, indicating that their function could be essential for cell growth. CDP-activated ribitol is a precursor for the synthesis of pneumococcal teichoic acids and some of the capsular polysaccharides. Thus, all eight genes in the lic region have a role in teichoic acid synthesis.

PubMed: 19074383
DOI: 10.1128/JB.01120-08

実験手法

X-RAY DIFFRACTION (2.75 Å)