2VQC

Structure of a DNA binding winged-helix protein, F-112, from Sulfolobus Spindle-shaped Virus 1.

「2CMX」から置き換えられました

2VQC の概要

• エントリーDOI: 10.2210/pdb2vqc/pdb
• 分子名称: HYPOTHETICAL 13.2 KDA PROTEIN (2 entities in total)
• 機能のキーワード: ssv, winged-helix, crenarchaeal, dna-binding protein, thermophilic protein, sulfolobus spindle virus, dna binding protein
• 由来する生物種: SULFOLOBUS VIRUS-LIKE PARTICLE SSV1
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 14191.57

構造登録者

Menon, S.K.,Kraft, P.,Corn, G.J.,Wiedenheft, B.,Young, M.J.,Lawrence, C.M. (登録日: 2008-03-12, 公開日: 2008-05-06, 最終更新日: 2024-11-20)

主引用文献

Menon, S.K.,Maaty, W.S.,Corn, G.J.,Kwok, S.C.,Eilers, B.J.,Kraft, P.,Gillitzer, E.,Young, M.J.,Bothner, B.,Lawrence, C.M.
Cysteine Usage in Sulfolobus Spindle-Shaped Virus 1 and Extension to Hyperthermophilic Viruses in General.
Virology, 376:270-, 2008

PubMed Abstract: Fuselloviridae are ubiquitous crenarchaeal viruses found in high-temperature acidic hot springs worldwide. The type virus, Sulfolobus spindle-shaped virus 1 (SSV1), has a double-stranded DNA genome that contains 34 open reading frames (ORFs). Fuselloviral genomes show little similarity to other organisms, generally precluding functional predictions. However, tertiary protein structure can provide insight into protein function. We have thus undertaken a systematic investigation of the SSV1 proteome and report here on the F112 gene product. Biochemical, proteomic and structural studies reveal a monomeric intracellular protein that adopts a winged helix DNA binding fold. Notably, the structure contains an intrachain disulfide bond, prompting analysis of cysteine usage in this and other hyperthermophilic viral genomes. The analysis supports a general abundance of disulfide bonds in the intracellular proteins of hyperthermophilic viruses, and reveals decreased cysteine content in the membrane proteins of hyperthermophilic viruses infecting Sulfolobales. The evolutionary implications of the SSV1 distribution are discussed.

PubMed: 18471851
DOI: 10.1016/J.VIROL.2008.03.026

実験手法

X-RAY DIFFRACTION (2.3 Å)