2VQ1

anti trimeric Lewis X Fab54-5C10-A

Summary for 2VQ1

• Entry DOI: 10.2210/pdb2vq1/pdb
• Descriptor: IGKV1-117 PROTEIN, ANTI-HUMAN FC GAMMA RECEPTOR III 3G8 GAMMA HEAVY CHAIN VARIABLE REGION, GLYCINE, ... (6 entities in total)
• Functional Keywords: monoclonal antibody, receptor, diagnosis, schistosomiasis, carbohydrate recognition, immune system
• Biological source: MUS MUSCULUS (MOUSE); More
• Total number of polymer chains: 4
• Total formula weight: 95700.76

Authors

de Geus, D.C.,van Roon, A.M.M.,Thomassen, E.A.J.,Hokke, C.H.,Deelder, A.M.,Abrahams, J.P. (deposition date: 2008-03-10, release date: 2009-01-27, Last modification date: 2024-11-20)

Primary citation

De Geus, D.C.,Van Roon, A.M.M.,Thomassen, E.A.J.,Hokke, C.H.,Deelder, A.M.,Abrahams, J.P.
Characterization of a Diagnostic Fab Fragment Binding Trimeric Lewis X.
Proteins, 76:439-, 2009

PubMed Abstract: Lewis X trisaccharides normally function as essential cell-cell interaction mediators. However, oligomers of Lewis X trisaccharides expressed by the parasite Schistosoma mansoni seem to be related to its evasion of the immune response of its human host. Here we show that monoclonal antibody 54-5C10-A, which is used to diagnose schistosomiasis in humans, interacts with oligomers of at least three Lewis X trisaccharides, but not with monomeric Lewis X. We describe the sequence and the 2.5 A crystal structure of its Fab fragment and infer a possible mode of binding of the polymeric Lewis X from docking studies. Our studies indicate a radically different mode of binding compared to Fab 291-2G3-A, which is specific for monomeric Lewis X, thus providing a structural explanation of the diagnostic success of 54-5C10-A.

PubMed: 19173313
DOI: 10.1002/PROT.22356

Experimental method

X-RAY DIFFRACTION (2.5 Å)