2VQ1
anti trimeric Lewis X Fab54-5C10-A
2VQ1 の概要
エントリーDOI | 10.2210/pdb2vq1/pdb |
分子名称 | IGKV1-117 PROTEIN, ANTI-HUMAN FC GAMMA RECEPTOR III 3G8 GAMMA HEAVY CHAIN VARIABLE REGION, GLYCINE, ... (6 entities in total) |
機能のキーワード | monoclonal antibody, receptor, diagnosis, schistosomiasis, carbohydrate recognition, immune system |
由来する生物種 | MUS MUSCULUS (MOUSE) 詳細 |
タンパク質・核酸の鎖数 | 4 |
化学式量合計 | 95700.76 |
構造登録者 | de Geus, D.C.,van Roon, A.M.M.,Thomassen, E.A.J.,Hokke, C.H.,Deelder, A.M.,Abrahams, J.P. (登録日: 2008-03-10, 公開日: 2009-01-27, 最終更新日: 2024-11-20) |
主引用文献 | De Geus, D.C.,Van Roon, A.M.M.,Thomassen, E.A.J.,Hokke, C.H.,Deelder, A.M.,Abrahams, J.P. Characterization of a Diagnostic Fab Fragment Binding Trimeric Lewis X. Proteins, 76:439-, 2009 Cited by PubMed Abstract: Lewis X trisaccharides normally function as essential cell-cell interaction mediators. However, oligomers of Lewis X trisaccharides expressed by the parasite Schistosoma mansoni seem to be related to its evasion of the immune response of its human host. Here we show that monoclonal antibody 54-5C10-A, which is used to diagnose schistosomiasis in humans, interacts with oligomers of at least three Lewis X trisaccharides, but not with monomeric Lewis X. We describe the sequence and the 2.5 A crystal structure of its Fab fragment and infer a possible mode of binding of the polymeric Lewis X from docking studies. Our studies indicate a radically different mode of binding compared to Fab 291-2G3-A, which is specific for monomeric Lewis X, thus providing a structural explanation of the diagnostic success of 54-5C10-A. PubMed: 19173313DOI: 10.1002/PROT.22356 主引用文献が同じPDBエントリー |
実験手法 | X-RAY DIFFRACTION (2.5 Å) |
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