2VL7

Structure of S. tokodaii Xpd4

2VL7 の概要

• エントリーDOI: 10.2210/pdb2vl7/pdb
• 分子名称: XPD, PHOSPHATE ION (3 entities in total)
• 機能のキーワード: helicase, unknown function
• 由来する生物種: SULFOLOBUS TOKODAII
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 62421.45

構造登録者

Naismith, J.H.,Johnson, K.A.,Oke, M.,McMahon, S.A.,Liu, L.,White, M.F.,Zawadski, M.,Carter, L.G. (登録日: 2008-01-08, 公開日: 2008-05-13, 最終更新日: 2024-11-20)

主引用文献

Liu, L.,Johnson, K.A.,Rudolf, J.,Mcrobbie, A.-M.,Mcmahon, S.A.,Oke, M.,Carter, L.G.,Naismith, J.H.,White, M.F.
Structure of the DNA Repair Helicase Xpd.
Cell(Cambridge,Mass.), 133:801-, 2008

PubMed Abstract: The XPD helicase (Rad3 in Saccharomyces cerevisiae) is a component of transcription factor IIH (TFIIH), which functions in transcription initiation and Nucleotide Excision Repair in eukaryotes, catalyzing DNA duplex opening localized to the transcription start site or site of DNA damage, respectively. XPD has a 5' to 3' polarity and the helicase activity is dependent on an iron-sulfur cluster binding domain, a feature that is conserved in related helicases such as FancJ. The xpd gene is the target of mutation in patients with xeroderma pigmentosum, trichothiodystrophy, and Cockayne's syndrome, characterized by a wide spectrum of symptoms ranging from cancer susceptibility to neurological and developmental defects. The 2.25 A crystal structure of XPD from the crenarchaeon Sulfolobus tokodaii, presented here together with detailed biochemical analyses, allows a molecular understanding of the structural basis for helicase activity and explains the phenotypes of xpd mutations in humans.

PubMed: 18510925
DOI: 10.1016/J.CELL.2008.04.029

実験手法

X-RAY DIFFRACTION (2.25 Å)