2VKR

3Fe-4S, 4Fe-4S plus Zn Acidianus ambivalens ferredoxin

Summary for 2VKR

• Entry DOI: 10.2210/pdb2vkr/pdb
• Descriptor: ZINC-CONTAINING FERREDOXIN, FE3-S4 CLUSTER, IRON/SULFUR CLUSTER, ... (5 entities in total)
• Functional Keywords: zinc, iron, 3fe-4s, 4fe-4s, transport, zn center, hemihedric twinnig, iron-sulfur protein, ferredoxin, methylation, thermophile, iron-sulfur, thermostable protein, metal-binding, protein folding, electron transport
• Biological source: ACIDIANUS AMBIVALENS
• Total number of polymer chains: 7
• Total formula weight: 82092.00

Authors

Frazao, C.,Aragao, D.,Coelho, R.,Leal, S.S.,Gomes, C.M.,Teixeira, M.,Carrondo, M.A. (deposition date: 2007-12-23, release date: 2008-03-04, Last modification date: 2024-02-07)

Primary citation

Frazao, C.,Aragao, D.,Coelho, R.,Leal, S.S.,Gomes, C.M.,Teixeira, M.,Carrondo, M.A.
Crystallographic analysis of the intact metal centres [3Fe-4S](1+/0) and [4Fe-4S](2+/1+) in a Zn(2+) -containing ferredoxin.
FEBS Lett., 582:763-767, 2008

PubMed Abstract: Detailed structural models of di-cluster seven-iron ferredoxins constitute a valuable resource for folding and stability studies relating the metal cofactors' role in protein stability. The here reported, hemihedric twinned crystal structure at 2.0 A resolution from Acidianus ambivalens ferredoxin, shows an integral 103 residues, physiologically relevant native form composed by a N-terminal extension comprising a His/Asp Zn(2+) site and the ferredoxin (betaalphabeta)(2) core, which harbours intact clusters I and II, a [3Fe-4S](1+/0) and a [4Fe-4S](2+/1+) centres. This is in contrast with the previously available ferredoxin structure from Sulfolofus tokodai, which was obtained from an artificial oxidative conversion with two [3Fe-4S](1+/0) centres and poor definition around cluster II.

PubMed: 18258200
DOI: 10.1016/j.febslet.2008.01.041

Experimental method

X-RAY DIFFRACTION (2.01 Å)