2VKR

3Fe-4S, 4Fe-4S plus Zn Acidianus ambivalens ferredoxin

2VKR の概要

• エントリーDOI: 10.2210/pdb2vkr/pdb
• 分子名称: ZINC-CONTAINING FERREDOXIN, FE3-S4 CLUSTER, IRON/SULFUR CLUSTER, ... (5 entities in total)
• 機能のキーワード: zinc, iron, 3fe-4s, 4fe-4s, transport, zn center, hemihedric twinnig, iron-sulfur protein, ferredoxin, methylation, thermophile, iron-sulfur, thermostable protein, metal-binding, protein folding, electron transport
• 由来する生物種: ACIDIANUS AMBIVALENS
• タンパク質・核酸の鎖数: 7
• 化学式量合計: 82092.00

構造登録者

Frazao, C.,Aragao, D.,Coelho, R.,Leal, S.S.,Gomes, C.M.,Teixeira, M.,Carrondo, M.A. (登録日: 2007-12-23, 公開日: 2008-03-04, 最終更新日: 2024-02-07)

主引用文献

Frazao, C.,Aragao, D.,Coelho, R.,Leal, S.S.,Gomes, C.M.,Teixeira, M.,Carrondo, M.A.
Crystallographic analysis of the intact metal centres [3Fe-4S](1+/0) and [4Fe-4S](2+/1+) in a Zn(2+) -containing ferredoxin.
FEBS Lett., 582:763-767, 2008

PubMed Abstract: Detailed structural models of di-cluster seven-iron ferredoxins constitute a valuable resource for folding and stability studies relating the metal cofactors' role in protein stability. The here reported, hemihedric twinned crystal structure at 2.0 A resolution from Acidianus ambivalens ferredoxin, shows an integral 103 residues, physiologically relevant native form composed by a N-terminal extension comprising a His/Asp Zn(2+) site and the ferredoxin (betaalphabeta)(2) core, which harbours intact clusters I and II, a [3Fe-4S](1+/0) and a [4Fe-4S](2+/1+) centres. This is in contrast with the previously available ferredoxin structure from Sulfolofus tokodai, which was obtained from an artificial oxidative conversion with two [3Fe-4S](1+/0) centres and poor definition around cluster II.

PubMed: 18258200
DOI: 10.1016/j.febslet.2008.01.041

実験手法

X-RAY DIFFRACTION (2.01 Å)