2VGB

HUMAN ERYTHROCYTE PYRUVATE KINASE

「1LIU」から置き換えられました

2VGB の概要

• エントリーDOI: 10.2210/pdb2vgb/pdb
• 関連するPDBエントリー: 1LIU 1LIW 1LIX 1LIY
• 分子名称: PYRUVATE KINASE ISOZYMES R/L, 1,6-di-O-phosphono-beta-D-fructofuranose, 2-PHOSPHOGLYCOLIC ACID, ... (6 entities in total)
• 機能のキーワード: metal-binding, phosphorylation, pyruvate kinase in the active r-state, kinase, pyruvate, magnesium, glycolysis, transferase, disease mutation
• 由来する生物種: HOMO SAPIENS (HUMAN)
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 230258.03

構造登録者

Valentini, G.,Chiarelli, L.,Fortin, R.,Dolzan, M.,Galizzi, A.,Abraham, D.J.,Wang, C.,Bianchi, P.,Zanella, A.,Mattevi, A. (登録日: 2007-11-12, 公開日: 2007-11-20, 最終更新日: 2024-05-01)

主引用文献

Valentini, G.,Chiarelli, L.R.,Fortin, R.,Dolzan, M.,Galizzi, A.,Abraham, D.J.,Wang, C.,Bianchi, P.,Zanella, A.,Mattevi, A.
Structure and Function of Human Erythrocyte Pyruvate Kinase. Molecular Basis of Nonspherocytic Hemolytic Anemia.
J.Biol.Chem., 277:23807-, 2002

PubMed Abstract: Deficiency of human erythrocyte isozyme (RPK) is, together with glucose-6-phosphate dehydrogenase deficiency, the most common cause of the nonspherocytic hemolytic anemia. To provide a molecular framework to the disease, we have solved the 2.7 A resolution crystal structure of human RPK in complex with fructose 1,6-bisphosphate, the allosteric activator, and phosphoglycolate, a substrate analogue, and we have functionally and structurally characterized eight mutants (G332S, G364D, T384M, D390N, R479H, R486W, R504L, and R532W) found in RPK-deficient patients. The mutations target distinct regions of RPK structure, including domain interfaces and catalytic and allosteric sites. The mutations affect to a different extent thermostability, catalytic efficiency, and regulatory properties. These studies are the first to correlate the clinical symptoms with the molecular properties of the mutant enzymes. Mutations greatly impairing thermostability and/or activity are associated with severe anemia. Some mutant proteins exhibit moderate changes in the kinetic parameters, which are sufficient to cause mild to severe anemia, underlining the crucial role of RPK for erythrocyte metabolism. Prediction of the effects of mutations is difficult because there is no relation between the nature and location of the replaced amino acid and the type of molecular perturbation. Characterization of mutant proteins may serve as a valuable tool to assist with diagnosis and genetic counseling.

PubMed: 11960989
DOI: 10.1074/JBC.M202107200

実験手法

X-RAY DIFFRACTION (2.73 Å)