2VE3

Retinoic acid bound cyanobacterial CYP120A1

2VE3 の概要

• エントリーDOI: 10.2210/pdb2ve3/pdb
• 関連するPDBエントリー: 2VE4
• 分子名称: PUTATIVE CYTOCHROME P450 120, PROTOPORPHYRIN IX CONTAINING FE, RETINOIC ACID, ... (4 entities in total)
• 機能のキーワード: oxidoreductase, monooxygenase, metal-binding, heme, iron
• 由来する生物種: SYNECHOCYSTIS SP.
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 103100.10

構造登録者

Kuhnel, K.,Ke, N.,Sligar, S.G.,Schuler, M.A.,Schlichting, I. (登録日: 2007-10-15, 公開日: 2008-04-29, 最終更新日: 2024-05-01)

主引用文献

Kuhnel, K.,Ke, N.,Cryle, M.J.,Sligar, S.G.,Schuler, M.A.,Schlichting, I.
Crystal Structures of Substrate-Free and Retinoic Acid-Bound Cyanobacterial Cytochrome P450 Cyp120A1.
Biochemistry, 47:6552-, 2008

PubMed Abstract: The crystal structures of substrate-free and all-trans-retinoic acid-bound CYP120A1 from Synechocystis sp. PCC 6803 were determined at 2.4 and 2.1 A resolution, respectively, representing the first structural characterization of a cyanobacterial P450. Features of CYP120A1 not observed in other P450 structures include an aromatic ladder flanking the channel leading to the active site and a triple-glycine motif within SRS5. Using spectroscopic methods, CYP120A1 is shown to bind 13-cis-retinoic acid, 9-cis-retinoic acid, and retinal with high affinity and dissociation constants of less than 1 microM. Metabolism of retinoic acid by CYP120A1 suggests that CYP120A1 hydroxylates a variety of retinoid derivatives in vivo. On the basis of the retinoic acid-bound CYP120A1 crystal structure, we propose that either carbon 2 or the methyl groups (C16 or C17) of the beta-ionone ring are modified by CYP120A1.

PubMed: 18512957
DOI: 10.1021/BI800328S

実験手法

X-RAY DIFFRACTION (2.1 Å)