2V91

STRUCTURE OF STRICTOSIDINE SYNTHASE IN COMPLEX WITH STRICTOSIDINE

2V91 の概要

• エントリーDOI: 10.2210/pdb2v91/pdb
• 関連するPDBエントリー: 2FP8 2FP9 2FPB 2FPC 2VAQ
• 分子名称: STRICTOSIDINE SYNTHASE, METHYL (2S,3R,4S)-3-ETHYL-2-(BETA-D-GLUCOPYRANOSYLOXY)-4-[(1S)-2,3,4,9-TETRAHYDRO-1H-BETA-CARBOLIN-1-YLMETHYL]-3,4-DIHYDRO-2H-PYRAN-5-CARBOXYLATE (3 entities in total)
• 機能のキーワード: alkaloid metabolism, six bladed beta propeller fold, str1, lyase, vacuole, synthase, glycoprotein
• 由来する生物種: RAUVOLFIA SERPENTINA (SERPENTWOOD)
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 68452.37

構造登録者

Loris, E.A.,Panjikar, S.,Ruppert, M.,Barleben, L.,Unger, M.,Stoeckigt, J. (登録日: 2007-08-16, 公開日: 2008-09-16, 最終更新日: 2024-11-06)

主引用文献

Loris, E.A.,Panjikar, S.,Ruppert, M.,Barleben, L.,Unger, M.,Schubel, H.,Stoeckigt, J.
Structure Based Engineering of Strictosidine Synthase: Auxiliary for Alkaloid Libraries
Chem.Biol., 14:979-, 2007

PubMed Abstract: The highly substrate-specific strictosidine synthase (EC 4.3.3.2) catalyzes the biological Pictet-Spengler condensation between tryptamine and secologanin, leading to the synthesis of about 2000 monoterpenoid indole alkaloids in higher plants. The crystal structure of Rauvolfia serpentina strictosidine synthase (STR1) in complex with strictosidine has been elucidated here, allowing the rational site-directed mutation of the active center of STR1 and resulting in modulation of its substrate acceptance. Here, we report on the rational redesign of STR1 by generation of a Val208Ala mutant, further describing the influence on substrate acceptance and the enzyme-catalyzed synthesis of 10-methyl- and 10-methoxystrictosidines. Based on the addition of strictosidine to a crude strictosidine glucosidase preparation from Catharanthus cells, a combined chemoenzymatic approach to generating large alkaloid libraries for future pharmacological screenings is presented.

PubMed: 17884630
DOI: 10.1016/J.CHEMBIOL.2007.08.009

実験手法

X-RAY DIFFRACTION (3.01 Å)