2V6W

tRNASer acceptor stem: Conformation and hydration of a microhelix in a crystal structure at 1.8 Angstrom resolution

2V6W の概要

• エントリーDOI: 10.2210/pdb2v6w/pdb
• 分子名称: 5'-R(*GP*GP*AP*GP*AP*GP*AP)-3', 5'-R(*UP*CP*UP*CP*UP*CP*CP)-3' (3 entities in total)
• 機能のキーワード: e.coli trnaser acceptor stem microhelix, nucleic acid trna identity, 1.8 angstrom resolution, hydration pattern, rna
• 由来する生物種: ESCHERICHIA COLI; 詳細
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 4417.75

構造登録者

Foerster, C.,Brauer, A.B.E.,Brode, S.,Fuerste, J.P.,Betzel, C.,Erdmann, V.A. (登録日: 2007-07-23, 公開日: 2007-11-06, 最終更新日: 2024-05-08)

主引用文献

Forster, C.,Brauer, A.B.E.,Brode, S.,Furste, J.P.,Betzel, C.,Erdmann, V.A.
Trnaser Acceptor Stem: Conformation and Hydration of a Microhelix in a Crystal Structure at 1.8 A Resolution.
Acta Crystallogr.,Sect.D, 63:1154-, 2007

PubMed Abstract: The crystal structure of a serine-specific tRNA acceptor-stem microhelix, the binding site for the seryl-tRNA synthetase, was solved by X-ray analysis. This seven-base-pair tRNA(Ser) microhelix forms endless rows of helices in the crystal lattice, with two helices stacking 'head-to-head' onto each other, resulting in an intermolecular guanosine stacking of the first purine nucleotides at the 5'-strands of the tRNA(Ser) microhelices. A network of 75 water loci could be associated with each RNA duplex. Unusual local geometric backbone parameters could be detected in the region of the G4 phosphate located in the 5'-strand of the helix, which lead to a ;kink' in this region and to an irregularly bent helix. The role of the specific hydration pattern and of the irregular conformation of the tRNA(Ser) acceptor-stem helix is discussed and summarized.

PubMed: 18007030
DOI: 10.1107/S0907444907045271

実験手法

X-RAY DIFFRACTION (1.8 Å)