2V5V

W57E Flavodoxin from Anabaena

2V5V の概要

• エントリーDOI: 10.2210/pdb2v5v/pdb
• 関連するPDBエントリー: 1DX9 1FTG 1OBO 1OBV 1QHE 2V5U
• 分子名称: FLAVODOXIN, FLAVIN MONONUCLEOTIDE, MAGNESIUM ION, ... (4 entities in total)
• 機能のキーワード: transport, electron transfer, electron transport
• 由来する生物種: ANABAENA SP.
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 38485.86

構造登録者

Herguedas, B.,Martinez-Julvez, M.,Perez-Dorado, I.,Goni, G.,Medina, M.,Hermoso, J.A. (登録日: 2007-07-10, 公開日: 2007-10-16, 最終更新日: 2023-12-13)

主引用文献

Frago, S.,Goni, G.,Herguedas, B.,Peregrina, J.R.,Serrano, A.,Perez-Dorado, I.,Molina, R.,Gomez-Moreno, C.,Hermoso, J.A.,Martinez-Julvez, M.,Mayhew, S.G.,Medina, M.
Tuning of the Fmn Binding and Oxido-Reduction Properties by Neighboring Side Chains in Anabaena Flavodoxin.
Arch.Biochem.Biophys., 467:206-, 2007

PubMed Abstract: Contribution of three regions (phosphate-binding, 50's and 90's loops) of Anabaena apoflavodoxin to FMN binding and reduction potential was studied. Thr12 and Glu16 did not influence FMN redox properties, but Thr12 played a role in FMN binding. Replacement of Trp57 with Glu, Lys or Arg moderately shifted E(ox/sq) and E(sq/hq) and altered the energetic of the FMN redox states binding profile. Our data indicate that the side chain of position 57 does not modulate E(ox/sq) by aromatic stacking or solvent exclusion, but rather by influencing the relative strength of the H-bond between the N(5) of the flavin and the Asn58-Ile59 bond. A correlation was observed between the isoalloxazine increase in solvent accessibility and less negative E(sq/hq). Moreover, E(sq/hq) became less negative as positively charged residues were added near to the isoalloxazine. Ile59 and Ile92 were simultaneously mutated to Ala or Glu. These mutations impaired FMN binding, while shifting E(sq/hq) to less negative values and E(ox/sq) to more negative. These effects are discussed on the bases of the X-ray structures of some of the Fld mutants, suggesting that in Anabaena Fld the structural control of both electron transfer steps is much more subtle than in other Flds.

PubMed: 17904516
DOI: 10.1016/J.ABB.2007.08.024

実験手法

X-RAY DIFFRACTION (1.88 Å)