2V4E

A non-cytotoxic DsRed variant for whole-cell labeling

Summary for 2V4E

• Entry DOI: 10.2210/pdb2v4e/pdb
• Related: 2VAE
• Descriptor: RED FLUORESCENT PROTEIN DRFP583, ... (4 entities in total)
• Functional Keywords: dsred, chromophore, luminescence, photoprotein, fluorescent protein
• Biological source: DISCOSOMA SP. (SEA ANEMONE 111); More
• Total number of polymer chains: 8
• Total formula weight: 201428.37

Authors

Strack, R.L.,Strongin, D.E.,Bhattacharyya, D.,Tao, W.,Berman, A.,Broxmeyer, H.E.,Keenan, R.J.,Glick, B.S. (deposition date: 2008-09-20, release date: 2008-11-04, Last modification date: 2023-12-13)

Primary citation

Strack, R.L.,Strongin, D.E.,Bhattacharyya, D.,Tao, W.,Berman, A.,Broxmeyer, H.E.,Keenan, R.J.,Glick, B.S.
A Noncytotoxic Dsred Variant for Whole-Cell Labeling.
Nat.Methods, 5:955-, 2008

PubMed Abstract: A common application of fluorescent proteins is to label whole cells, but many RFPs are cytotoxic when used with standard high-level expression systems. We engineered a rapidly maturing tetrameric fluorescent protein called DsRed-Express2 that has minimal cytotoxicity. DsRed-Express2 exhibits strong and stable expression in bacterial and mammalian cells, and it outperforms other available RFPs with regard to photostability and phototoxicity.

PubMed: 18953349
DOI: 10.1038/NMETH.1264

Experimental method

X-RAY DIFFRACTION (2.4 Å)