2V4E

A non-cytotoxic DsRed variant for whole-cell labeling

2V4E の概要

• エントリーDOI: 10.2210/pdb2v4e/pdb
• 関連するPDBエントリー: 2VAE
• 分子名称: RED FLUORESCENT PROTEIN DRFP583, ... (4 entities in total)
• 機能のキーワード: dsred, chromophore, luminescence, photoprotein, fluorescent protein
• 由来する生物種: DISCOSOMA SP. (SEA ANEMONE 111); 詳細
• タンパク質・核酸の鎖数: 8
• 化学式量合計: 201428.37

構造登録者

Strack, R.L.,Strongin, D.E.,Bhattacharyya, D.,Tao, W.,Berman, A.,Broxmeyer, H.E.,Keenan, R.J.,Glick, B.S. (登録日: 2008-09-20, 公開日: 2008-11-04, 最終更新日: 2023-12-13)

主引用文献

Strack, R.L.,Strongin, D.E.,Bhattacharyya, D.,Tao, W.,Berman, A.,Broxmeyer, H.E.,Keenan, R.J.,Glick, B.S.
A Noncytotoxic Dsred Variant for Whole-Cell Labeling.
Nat.Methods, 5:955-, 2008

PubMed Abstract: A common application of fluorescent proteins is to label whole cells, but many RFPs are cytotoxic when used with standard high-level expression systems. We engineered a rapidly maturing tetrameric fluorescent protein called DsRed-Express2 that has minimal cytotoxicity. DsRed-Express2 exhibits strong and stable expression in bacterial and mammalian cells, and it outperforms other available RFPs with regard to photostability and phototoxicity.

PubMed: 18953349
DOI: 10.1038/NMETH.1264

実験手法

X-RAY DIFFRACTION (2.4 Å)