2V43

Crystal structure of RseB: a sensor for periplasmic stress response in E. coli

2V43 の概要

• エントリーDOI: 10.2210/pdb2v43/pdb
• 関連するPDBエントリー: 2V42
• 分子名称: SIGMA-E FACTOR REGULATORY PROTEIN RSEB, CYSTEINE (3 entities in total)
• 機能のキーワード: sensor for periplasmic stress, regulator, regulatory protein, lipoprotein binding
• 由来する生物種: ESCHERICHIA COLI
• 細胞内の位置: Periplasm (Potential): P0AFX9
• タンパク質・核酸の鎖数: 3
• 化学式量合計: 103564.31

構造登録者

Wollmann, P.,Zeth, K. (登録日: 2007-06-27, 公開日: 2007-08-28, 最終更新日: 2024-05-01)

主引用文献

Wollmann, P.,Zeth, K.
The Structure of Rseb: A Sensor in Periplasmic Stress Response of E. Coli.
J.Mol.Biol., 372:927-, 2007

PubMed Abstract: An elegant network of signal transduction has evolved in the bacterial cell envelope to respond to environmental stress. It is initiated by sensing unfavourable and harmful changes in the periplasm. The stress signal is then transmitted by a controlled degradation of the transmembrane anti-sigma-factor RseA that leads to the activation of the alternative sigma factor sigma(E). The periplasmic protein RseB exerts a crucial role in modulating the stability of RseA. RseB from Escherichia coli has been crystallized and crystal structures were determined at 2.4 A and at 2.8 A resolution. The protein forms a homodimer, with the monomer composed of two domains. The large domain resembles an unclosed beta-barrel that is structurally remarkably similar to a protein family capable of binding the lipid anchor of lipoproteins. The small C-terminal domain, connected to the large domain by a partially unstructured loop, is responsible for interaction with RseA. On the basis of the structure of RseB, we suggest that it acts as a sensor of periplasmic stress with a dual functionality: it detects mislocalized lipoproteins and propagates the signal to induce the sigma(E)-response.

PubMed: 17692869
DOI: 10.1016/J.JMB.2007.06.039

実験手法

X-RAY DIFFRACTION (2.37 Å)