2V3Q

Serendipitous discovery and X-ray structure of a human phosphate binding apolipoprotein

「2CAP」から置き換えられました

2V3Q の概要

• エントリーDOI: 10.2210/pdb2v3q/pdb
• 分子名称: HUMAN PHOSPHATE BINDING PROTEIN, GLYCEROL, PHOSPHATE ION, ... (5 entities in total)
• 機能のキーワード: atherosclerosis, hdl, missing gene, paraoxonase, phosphatemia, transport protein, transporter
• 由来する生物種: HOMO SAPIENS (HUMAN)
• 細胞内の位置: Secreted : P85173
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 39771.22

構造登録者

Morales, R.,Berna, A.,Carpentier, P.,Elias, M.,Contreras-Martel, C.,Renault, F.,Nicodeme, M.,Chesne-Seck, M.-L.,Bernier, F.,Dupuy, J.,Schaeffer, C.,Diemer, H.,Van Dorsselaer, A.,Fontecilla, J.C.,Masson, P.,Rochu, D.,Chabriere, E. (登録日: 2007-06-20, 公開日: 2008-07-22, 最終更新日: 2024-10-23)

主引用文献

Diemer, H.,Elias, M.,Renault, F.,Rochu, D.,Contreras-Martel, C.,Schaeffer, C.,Van Dorsselaer, A.,Chabriere, E.
Tandem Use of X-Ray Crystallography and Mass Spectrometry to Obtain Ab Initio the Complete and Exact Amino Acids Sequence of Hpbp, a Human 38kDa Apolipoprotein
Proteins: Struct., Funct., Bioinf., 71:1708-, 2008

PubMed Abstract: The Human Phosphate Binding Protein (HPBP) is a serendipitously discovered apolipoprotein from human plasma that binds phosphate. Amino acid sequence relates HPBP to an intriguing protein family that seems ubiquitous in eukaryotes. These proteins, named DING according to the sequence of their four conserved N-terminal residues, are systematically absent from eukaryotic genome databases. As a consequence, HPBP amino acids sequence had to be first assigned from the electronic density map. Then, an original approach combining X-ray crystallography and mass spectrometry provides the complete and a priori exact sequence of the 38-kDa HPBP. This first complete sequence of a eukaryotic DING protein will be helpful to study HPBP and the entire DING protein family.

PubMed: 18076037
DOI: 10.1002/PROT.21866

実験手法

X-RAY DIFFRACTION (1.89 Å)