2V3M

Structure of the Gar1 domain of NAf1

2V3M の概要

• エントリーDOI: 10.2210/pdb2v3m/pdb
• 分子名称: NAF1, SULFATE ION (2 entities in total)
• 機能のキーワード: ribosomal protein, naf1, gar1, snornp, phosphorylation, hypothetical protein
• 由来する生物種: SACCHAROMYCES CEREVISIAE (BAKER'S YEAST)
• 細胞内の位置: Nucleus: P53919
• タンパク質・核酸の鎖数: 6
• 化学式量合計: 92077.06

構造登録者

Leulliot, N.,Godin, K.S.,Hoareau-Aveilla, C.,Quevillon-Cheruel, S.,Varani, G.,Henry, Y.,van Tilbeurgh, H. (登録日: 2007-06-19, 公開日: 2007-07-10, 最終更新日: 2024-11-06)

主引用文献

Leulliot, N.,Godin, K.S.,Hoareau-Aveilla, C.,Quevillon-Cheruel, S.,Varani, G.,Henry, Y.,Van Tilbeurgh, H.
The Box H/Aca Rnp Assembly Factor Naf1P Contains a Domain Homologous to Gar1P Mediating its Interaction with Cbf5P.
J.Mol.Biol., 371:1338-, 2007

PubMed Abstract: Naf1 is an essential protein involved in the maturation of box H/ACA ribonucleoproteins, a group of particles required for ribosome biogenesis, modification of spliceosomal small nuclear RNAs and telomere synthesis. Naf1 participates in the assembly of the RNP at transcription sites and in the nuclear trafficking of the complex. The crystal structure of a domain of yeast Naf1p, Naf1Delta1p, reveals a striking structural homology with the core domain of archaeal Gar1, an essential protein component of the mature RNP; it suggests that Naf1p and Gar1p have a common binding site on the enzymatic protein component of the particle, Cbf5p. We propose that Naf1p is a competitive binder for Cbf5p, which is replaced by Gar1p during maturation of the H/ACA particle. The exchange of Naf1p by Gar1p might be prompted by external factors that alter the oligomerisation state of Naf1p and Gar1p. The structural homology with Gar1 suggests that the function of Naf1 involves preventing non-cognate RNAs from being loaded during transport of the particle by inducing a non-productive conformation of Cbf5.

PubMed: 17612558
DOI: 10.1016/J.JMB.2007.06.031

実験手法

X-RAY DIFFRACTION (2.74 Å)