2UYT

Structure of L-rhamnulose kinase in complex with ADP and beta-L- rhamnulose.

2UYT の概要

• エントリーDOI: 10.2210/pdb2uyt/pdb
• 関連するPDBエントリー: 2CGJ 2CGK 2CGL
• 分子名称: RHAMNULOKINASE, 6-deoxy-beta-L-fructofuranose, ADENOSINE-5'-DIPHOSPHATE, ... (4 entities in total)
• 機能のキーワード: rhamnose degradation, in-line phosphoryl transfer, hexokinase-hsp70-actin superfamily, l-rhamnulose kinase, rhamnose metabolism, kinase, induced fit, transferase, rare sugars
• 由来する生物種: ESCHERICHIA COLI BL21(DE3)
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 54546.07

構造登録者

Grueninger, D.,Schulz, G.E. (登録日: 2007-04-13, 公開日: 2007-06-26, 最終更新日: 2024-10-09)

主引用文献

Grueninger, D.,Schulz, G.E.
Substrate Spectrum of L-Rhamnulose Kinase Related to Models Derived from Two Ternary Complex Structures.
FEBS Lett., 581:3127-, 2007

PubMed Abstract: The enzyme L-rhamnulose kinase from Escherichia coli participates in the degradation pathway of L-rhamnose, a common natural deoxy-hexose. The structure of the enzyme in a ternary complex with its substrates ADP and L-rhamnulose has been determined at 1.55A resolution and refined to R(cryst)/R(free) values of 0.179/0.209. The result was compared with the lower resolution structure of a corresponding complex containing L-fructose instead of L-rhamnulose. In light of the two established sugar positions and conformations, a number of rare sugars have been modeled into the active center of L-rhamnulose kinase and the model structures have been compared with the known enzymatic phosphorylation rates. Rare sugars are of rising interest for the synthesis of bioactive compounds.

PubMed: 17568582
DOI: 10.1016/J.FEBSLET.2007.05.075

実験手法

X-RAY DIFFRACTION (1.55 Å)