2UY7

Crystal structure of the P pilus rod subunit PapA

2UY7 の概要

• エントリーDOI: 10.2210/pdb2uy7/pdb
• 関連するPDBエントリー: 1N0L 1PDK 1QPP 1QPX 2J2Z 2J7L 2UY6 3DPA
• 分子名称: PERIPLASMID CHAPERONE PAPD PROTEIN, PAP FIMBRIAL MAJOR PILIN PROTEIN, SULFATE ION, ... (4 entities in total)
• 機能のキーワード: donor strand complementation, pili/n-terminal extension, pilus biogenesis, donor-strand exchange, nte, dsc, dse, papa, papd, fimbria, chaperone
• 由来する生物種: ESCHERICHIA COLI; 詳細
• 細胞内の位置: Periplasm (By similarity): Q1R2W9; Secreted: P04127
• タンパク質・核酸の鎖数: 8
• 化学式量合計: 166109.98

構造登録者

Verger, D.,Bullitt, E.,Hultgren, S.J.,Waksman, G. (登録日: 2007-04-02, 公開日: 2007-05-29, 最終更新日: 2024-11-13)

主引用文献

Verger, D.,Bullitt, E.,Hultgren, S.J.,Waksman, G.
Crystal Structure of the P Pilus Rod Subunit Papa.
Plos Pathog., 3:E73-, 2007

PubMed Abstract: P pili are important adhesive fibres involved in kidney infection by uropathogenic Escherichia coli strains. P pili are assembled by the conserved chaperone-usher pathway, which involves the PapD chaperone and the PapC usher. During pilus assembly, subunits are incorporated into the growing fiber via the donor-strand exchange (DSE) mechanism, whereby the chaperone's G1 beta-strand that complements the incomplete immunoglobulin-fold of each subunit is displaced by the N-terminal extension (Nte) of an incoming subunit. P pili comprise a helical rod, a tip fibrillum, and an adhesin at the distal end. PapA is the rod subunit and is assembled into a superhelical right-handed structure. Here, we have solved the structure of a ternary complex of PapD bound to PapA through donor-strand complementation, itself bound to another PapA subunit through DSE. This structure provides insight into the structural basis of the DSE reaction involving this important pilus subunit. Using gel filtration chromatography and electron microscopy on a number of PapA Nte mutants, we establish that PapA differs in its mode of assembly compared with other Pap subunits, involving a much larger Nte that encompasses not only the DSE region of the Nte but also the region N-terminal to it.

PubMed: 17511517
DOI: 10.1371/JOURNAL.PPAT.0030073

実験手法

X-RAY DIFFRACTION (2.6 Å)