2UXE

The structure of Vaccinia virus N1

2UXE の概要

• エントリーDOI: 10.2210/pdb2uxe/pdb
• 関連するPDBエントリー: 2I39
• 分子名称: HYPOTHETICAL PROTEIN (1 entity in total)
• 機能のキーワード: viral protein, bcl-like protein, hypothetical protein, viral protein apoptosis, vaccinia virus, virulence factor
• 由来する生物種: VACCINIA VIRUS
• タンパク質・核酸の鎖数: 6
• 化学式量合計: 85741.44

構造登録者

Cooray, S.,Bahar, M.W.,Abrescia, N.G.A.,McVey, C.E.,Bartlett, N.W.,Chen, R.A.-J.,Stuart, D.I.,Grimes, J.M.,Smith, G.L. (登録日: 2007-03-28, 公開日: 2007-05-22, 最終更新日: 2024-11-13)

主引用文献

Cooray, S.,Bahar, M.W.,Abrescia, N.G.A.,Mcvey, C.E.,Bartlett, N.W.,Chen, R.A.-J.,Stuart, D.I.,Grimes, J.M.,Smith, G.L.
Functional and Structural Studies of the Vaccinia Virus Virulence Factor N1 Reveal a Bcl-2-Like Anti- Apoptotic Protein
J.Gen.Virol., 88:1656-, 2007

PubMed Abstract: Vaccinia virus (VACV) encodes many immunomodulatory proteins, including inhibitors of apoptosis and modulators of innate immune signalling. VACV protein N1 is an intracellular homodimer that contributes to virus virulence and was reported to inhibit nuclear factor (NF)-kappaB signalling. However, analysis of NF-kappaB signalling in cells infected with recombinant viruses with or without the N1L gene showed no difference in NF-kappaB-dependent gene expression. Given that N1 promotes virus virulence, other possible functions of N1 were investigated and this revealed that N1 is an inhibitor of apoptosis in cells transfected with the N1L gene and in the context of VACV infection. In support of this finding virally expressed N1 co-precipitated with endogenous pro-apoptotic Bcl-2 proteins Bid, Bad and Bax as well as with Bad and Bax expressed by transfection. In addition, the crystal structure of N1 was solved to 2.9 A resolution (0.29 nm). Remarkably, although N1 shows no sequence similarity to cellular proteins, its three-dimensional structure closely resembles Bcl-x(L) and other members of the Bcl-2 protein family. The structure also reveals that N1 has a constitutively open surface groove similar to the grooves of other anti-apoptotic Bcl-2 proteins, which bind the BH3 motifs of pro-apoptotic Bcl-2 family members. Molecular modelling of BH3 peptides into the N1 surface groove, together with analysis of their physico-chemical properties, suggests a mechanism for the specificity of peptide recognition. This study illustrates the importance of the evolutionary conservation of structure, rather than sequence, in protein function and reveals a novel anti-apoptotic protein from orthopoxviruses.

PubMed: 17485524
DOI: 10.1099/VIR.0.82772-0

実験手法

X-RAY DIFFRACTION (2.9 Å)