2UXD

Crystal structure of an extended tRNA anticodon stem loop in complex with its cognate mRNA CGGG in the context of the Thermus thermophilus 30S subunit.

2UXD の概要

• エントリーDOI: 10.2210/pdb2uxd/pdb
• 関連するPDBエントリー: 1FJG 1GIX 1I94 1I95 1I96 1I97 1IBK 1IBL 1IBM 1J5E 1JGO 1JGP 1JGQ 1L1U 1N32 1N33 1N34 1N36 1PNS 1PNX 1XMO 1XMQ 1XNQ 1XNR 1YL4 2B64 2B9M 2B9O 2F4V 2J00 2J02 2UU9 2UUA 2UUB 2UUC 2UXB 2UXC
• 分子名称: 16S RIBOSOMAL RNA, RIBOSOMAL PROTEIN S10, RIBOSOMAL PROTEIN S11, ... (27 entities in total)
• 機能のキーワード: ribonucleoprotein, 30s ribosomal subunit, frameshift suppressor trna, trna, mrna, codon, a site, ribosome, decoding, metal-binding, messenger rna, ribosomal protein, rna-binding, paromomycin, anticodon, stem-loop, frameshift, zinc-finger, rrna-binding, trna-binding, transfer rna
• 由来する生物種: THERMUS THERMOPHILUS; 詳細
• タンパク質・核酸の鎖数: 23
• 化学式量合計: 793791.12

構造登録者

Dunham, C.M.,Selmer, M.,Phelps, S.S.,Kelley, A.C.,Suzuki, T.,Joseph, S.,Ramakrishnan, V. (登録日: 2007-03-28, 公開日: 2007-10-02, 最終更新日: 2024-10-16)

主引用文献

Dunham, C.M.,Selmer, M.,Phelps, S.S.,Kelley, A.C.,Suzuki, T.,Joseph, S.,Ramakrishnan, V.
Structures of Trnas with an Expanded Anticodon Loop in the Decoding Center of the 30S Ribosomal Subunit.
RNA, 13:817-, 2007

PubMed Abstract: During translation, some +1 frameshift mRNA sites are decoded by frameshift suppressor tRNAs that contain an extra base in their anticodon loops. Similarly engineered tRNAs have been used to insert nonnatural amino acids into proteins. Here, we report crystal structures of two anticodon stem-loops (ASLs) from tRNAs known to facilitate +1 frameshifting bound to the 30S ribosomal subunit with their cognate mRNAs. ASL(CCCG) and ASL(ACCC) (5'-3' nomenclature) form unpredicted anticodon-codon interactions where the anticodon base 34 at the wobble position contacts either the fourth codon base or the third and fourth codon bases. In addition, we report the structure of ASL(ACGA) bound to the 30S ribosomal subunit with its cognate mRNA. The tRNA containing this ASL was previously shown to be unable to facilitate +1 frameshifting in competition with normal tRNAs (Hohsaka et al. 2001), and interestingly, it displays a normal anticodon-codon interaction. These structures show that the expanded anticodon loop of +1 frameshift promoting tRNAs are flexible enough to adopt conformations that allow three bases of the anticodon to span four bases of the mRNA. Therefore it appears that normal triplet pairing is not an absolute constraint of the decoding center.

PubMed: 17416634
DOI: 10.1261/RNA.367307

実験手法

X-RAY DIFFRACTION (3.2 Å)