2UAG

UDP-N-ACETYLMURAMOYL-L-ALANINE:D-GLUTAMATE LIGASE

2UAG の概要

• エントリーDOI: 10.2210/pdb2uag/pdb
• 分子名称: PROTEIN (UDP-N-ACETYLMURAMOYL-L-ALANINE:D-GLUTAMATE LIGASE), MAGNESIUM ION, URIDINE-5'-DIPHOSPHATE-N-ACETYLMURAMOYL-L-ALANINE, ... (5 entities in total)
• 機能のキーワード: ligase, peptidoglycan synthesis, murd, adp-forming enzyme
• 由来する生物種: Escherichia coli
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 48158.56

構造登録者

Bertrand, J.,Fanchon, E.,Dideberg, O. (登録日: 1999-02-23, 公開日: 2000-02-25, 最終更新日: 2023-12-27)

主引用文献

Bertrand, J.A.,Auger, G.,Martin, L.,Fanchon, E.,Blanot, D.,Le Beller, D.,van Heijenoort, J.,Dideberg, O.
Determination of the MurD mechanism through crystallographic analysis of enzyme complexes.
J.Mol.Biol., 289:579-590, 1999

PubMed Abstract: UDP -N- acetylmuramoyl- L -alanine: D -glutamate (MurD) ligase catalyses the addition of d -glutamate to the nucleotide precursor UDP -N- acetylmuramoyl- L -alanine (UMA). The crystal structures of three complexes of Escherichia coli MurD with a variety of substrates and products have been determined to high resolution. These include (1) the quaternary complex of MurD, the substrate UMA, the product ADP, and Mg2+, (2) the quaternary complex of MurD, the substrate UMA, the product ADP, and Mn2+, and (3) the binary complex of MurD with the product UDP - N- acetylmuramoyl- L -alanine- D -glutamate (UMAG). The reaction mechanism supported by these structures proceeds by the phosphorylation of the C-terminal carboxylate group of UMA by the gamma-phosphate group of ATP to form an acyl-phosphate intermediate, followed by the nucleophilic attack by the amino group of D-glutamate to produce UMAG. A key feature in the reaction intermediate is the presence of two magnesium ions bridging negatively charged groups.

PubMed: 10356330
DOI: 10.1006/jmbi.1999.2800

実験手法

X-RAY DIFFRACTION (1.7 Å)