2SPT

DIFFERENCES IN THE METAL ION STRUCTURE BETWEEN SR-AND CA-PROTHROMBIN FRAGMENT 1

2SPT の概要

• エントリーDOI: 10.2210/pdb2spt/pdb
• 分子名称: PROTHROMBIN, 2-acetamido-2-deoxy-beta-D-glucopyranose, STRONTIUM ION, ... (4 entities in total)
• 機能のキーワード: hydrolase(serine proteinase)
• 由来する生物種: Bos taurus (cattle)
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 17651.35

構造登録者

Tulinsky, A. (登録日: 1994-02-01, 公開日: 1994-05-31, 最終更新日: 2025-03-26)

主引用文献

Seshadri, T.P.,Skrzypczak-Jankun, E.,Yin, M.,Tulinsky, A.
Differences in the metal ion structure between Sr- and Ca-prothrombin fragment 1.
Biochemistry, 33:1087-1092, 1994

PubMed Abstract: The structure of Sr-prothrombin fragment 1 has been solved and refined by restrained least-squares methods at 2.5-A resolution to a crystallographic R value of 0.167. The protein structure is very similar to that of Ca-fragment 1. A polymeric array of five Sr2+ ions separated by about 4.0 A is buried among six gamma-carboxyglutamic acid (Gla) residues; three other Sr2+ ions interact with other Gla residues and are located further apart. One of these was not found in the Ca-fragment 1 structure. The coordination of the Sr2+ ions resembles that of Ca2+, but there are some significant differences between them. The most notable is the lack of water coordination with Sr2+ ions and two conformations for Gla 8, which change the coordination of Sr-2 and Sr-3. A hexose moiety of an oligosaccharide was located in the vicinity of Asn101 that was flexibly disordered in Ca-fragment 1. The new Sr2+ ion found may be involved in metal ion phospholipid binding interactions along with Sr-1, and Sr-7, Sr-8.

PubMed: 8110739
DOI: 10.1021/bi00171a006

実験手法

X-RAY DIFFRACTION (2.5 Å)