Loading
PDBj
MenuPDBj@FacebookPDBj@X(formerly Twitter)PDBj@BlueSkyPDBj@YouTubewwPDB FoundationwwPDBDonate
RCSB PDBPDBeBMRBAdv. SearchSearch help

2RSK

RNA aptamer against prion protein in complex with the partial binding peptide

Summary for 2RSK
Entry DOI10.2210/pdb2rsk/pdb
DescriptorRNA (5'-R(*GP*GP*AP*GP*GP*AP*GP*GP*AP*GP*GP*A)-3'), partial binding peptide of Major prion protein (2 entities in total)
Functional Keywordsaptamer, prion, rna, alzheimer's disease, membrane protein-rna complex, membrane protein/rna
Biological sourceBos taurus (Bovine)
Cellular locationCell membrane; Lipid-anchor, GPI-anchor (By similarity): P10279
Total number of polymer chains4
Total formula weight10842.15
Authors
Mashima, T.,Nishikawa, F.,Kamatari, Y.O.,Fujiwara, H.,Nishikawa, S.,Kuwata, K.,Katahira, M. (deposition date: 2012-03-08, release date: 2013-02-13, Last modification date: 2024-05-15)
Primary citationMashima, T.,Nishikawa, F.,Kamatari, Y.O.,Fujiwara, H.,Saimura, M.,Nagata, T.,Kodaki, T.,Nishikawa, S.,Kuwata, K.,Katahira, M.
Anti-prion activity of an RNA aptamer and its structural basis
Nucleic Acids Res., 41:1355-1362, 2013
Cited by
PubMed Abstract: Prion proteins (PrPs) cause prion diseases, such as bovine spongiform encephalopathy. The conversion of a normal cellular form (PrP(C)) of PrP into an abnormal form (PrP(Sc)) is thought to be associated with the pathogenesis. An RNA aptamer that tightly binds to and stabilizes PrP(C) is expected to block this conversion and to thereby prevent prion diseases. Here, we show that an RNA aptamer comprising only 12 residues, r(GGAGGAGGAGGA) (R12), reduces the PrP(Sc) level in mouse neuronal cells persistently infected with the transmissible spongiform encephalopathy agent. Nuclear magnetic resonance analysis revealed that R12, folded into a unique quadruplex structure, forms a dimer and that each monomer simultaneously binds to two portions of the N-terminal half of PrP(C), resulting in tight binding. Electrostatic and stacking interactions contribute to the affinity of each portion. Our results demonstrate the therapeutic potential of an RNA aptamer as to prion diseases.
PubMed: 23180780
DOI: 10.1093/nar/gks1132
PDB entries with the same primary citation
Experimental method
SOLUTION NMR
Structure validation

239803

数据于2025-08-06公开中

PDB statisticsPDBj update infoContact PDBjnumon