2ROT

Structure of chimeric variant of SH3 domain- SHH

Summary for 2ROT

• Entry DOI: 10.2210/pdb2rot/pdb
• NMR Information: BMRB: 11043
• Descriptor: Spectrin alpha chain, brain (1 entity in total)
• Functional Keywords: sh3, chimeric protein, alpha-spectrin, actin capping, actin-binding, calcium, calmodulin-binding, cytoplasm, cytoskeleton, phosphoprotein, sh3 domain, protein binding
• Biological source: Gallus gallus (Chicken)
• Total number of polymer chains: 1
• Total formula weight: 8136.35

Authors

Kutyshenko, N.P.,Prokhorov, D.A.,Timchenko, M.A.,Kudrevatykh, Y.A.,Gushchina, L.V.,Khristoforov, V.S.,Filimonov, V.V. (deposition date: 2008-04-10, release date: 2009-04-28, Last modification date: 2024-05-15)

Primary citation

Kutyshenko, V.P.,Prokhorov, D.A.,Timchenko, M.A.,Kudrevatykh, Y.A.,Gushchina, L.V.,Khristoforov, V.S.,Filimonov, V.V.,Uversky, V.N.
Solution structure and dynamics of the chimeric SH3 domains, SHH- and SHA-"Bergeracs".
Biochim.Biophys.Acta, 1794:1813-1822, 2009

PubMed Abstract: Two chimeric proteins, SHcapital EN, Cyrillic and SHA of the "SH3-Bergerac" family (where the beta-turn N47D48 in spectrin SH3 domain was substituted for KITVNGKTYE or KATANGKTYE sequences, respectively), were analyzed by high-resolution NMR to resolve their spatial structures and to analyze their dynamics. Although the presence of a stable beta-hairpin in the region of the insertion was confirmed, the introduced extension of the polypeptide chain in SHcapital EN, Cyrillic (approximately 17%) practically did not affect the total molecule topology. Interestingly, the introduced beta-hairpin had higher mobility in comparison with other protein regions. Finally, we performed a disorder prediction with the PONDR VSL2 algorithm and discovered that the inserted beta-hairpin in both SHH and SHA proteins exhibited significant propensity for intrinsic disorder and therefore for high mobility. In agreement with the experimental data, the predisposition for the increased intramolecular mobility was noticeably higher in SHA.

PubMed: 19732853
DOI: 10.1016/j.bbapap.2009.08.021

Experimental method

SOLUTION NMR