2RNX

The Structural Basis for Site-Specific Lysine-Acetylated Histone Recognition by the Bromodomains of the HUman Transcriptional Co-Activators PCAF and CBP

2RNX の概要

• エントリーDOI: 10.2210/pdb2rnx/pdb
• 関連するPDBエントリー: 2RNW
• 分子名称: Histone acetyltransferase PCAF, Histone H3 (2 entities in total)
• 機能のキーワード: bromodomain, histone, acetyltransferase, acyltransferase, cell cycle, host-virus interaction, nucleus, polymorphism, transcription, transcription regulation, acetylation, chromosomal protein, dna damage, dna repair, dna-binding, methylation, nucleosome core, phosphoprotein, transferase-nuclear protein complex, transferase/nuclear protein
• 由来する生物種: Homo sapiens (human); 詳細
• 細胞内の位置: Nucleus (By similarity): Q92831; Nucleus: P61830
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 15559.00

構造登録者

Zeng, L.,Zhang, Q.,Gerona-Navarro, G.,Zhou, M.M. (登録日: 2008-02-03, 公開日: 2008-05-06, 最終更新日: 2024-11-13)

主引用文献

Zeng, L.,Zhang, Q.,Gerona-Navarro, G.,Moshkina, N.,Zhou, M.M.
Structural Basis of Site-Specific Histone Recognition by the Bromodomains of Human Coactivators PCAF and CBP/p300
Structure, 16:643-652, 2008

PubMed Abstract: Histone lysine acetylation is central to epigenetic control of gene transcription. Bromodomains of chromosomal proteins function as acetyl-lysine (Kac) binding domains. However, how bromodomains recognize site-specific histones remains unanswered. Here, we report three three-dimensional solution structures of the bromodomains of the human transcriptional coactivators CREB-binding protein (CBP) and p300/CBP-associated factor (PCAF) bound to peptides derived from histone acetylation sites at lysines 36 and 9 in H3, and lysine 20 in H4. From structural and biochemical binding analyses, we determine consensus histone recognition by the bromodomains of PCAF and CBP, which represent two different subgroups of the bromodomain family. Through bromodomain residues in the ZA and BC loops, PCAF prefers acetylation sites with a hydrophobic residue at (Kac+2) position and a positively charged or aromatic residue at (Kac+3), whereas CBP favors bulky hydrophobic residues at (Kac+1) and (Kac+2), a positively charged residue at (Kac-1), and an aromatic residue at (Kac-2).

PubMed: 18400184
DOI: 10.1016/j.str.2008.01.010

実験手法

SOLUTION NMR