2RHP

The Thrombospondin-1 Polymorphism Asn700Ser Associated with Cornoary Artery Disease Causes Local and Long-Ranging Changes in Protein Structure

2RHP の概要

• エントリーDOI: 10.2210/pdb2rhp/pdb
• 関連するPDBエントリー: 1YO8
• 分子名称: Thrombospondin-2, 2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose, 2-acetamido-2-deoxy-beta-D-glucopyranose, ... (5 entities in total)
• 機能のキーワード: extracellular matrix, calcium-binding, glycosylated, jelly-roll, egf-like, lectin-like, cell adhesion, egf-like domain, glycoprotein, heparin-binding
• 由来する生物種: Homo sapiens (Human)
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 71368.64

構造登録者

Carlson, C.B.,Keck, J.L.,Mosher, D.F. (登録日: 2007-10-09, 公開日: 2008-05-27, 最終更新日: 2024-10-09)

主引用文献

Carlson, C.B.,Liu, Y.,Keck, J.L.,Mosher, D.F.
Influences of the N700S Thrombospondin-1 Polymorphism on Protein Structure and Stability.
J.Biol.Chem., 283:20069-20076, 2008

PubMed Abstract: Thrombospondins (THBSs) are multimodular, secreted proteins characterized by a signature domain comprising a unique set of 13 calcium-binding repeats flanked by epidermal growth factor (EGF)-like and lectin-like modules. A polymorphism that changes a conserved Asn to Ser at residue 700 in the most N-terminal calcium-binding repeat of THBS-1 (repeat 1C) is found in 8-10% of European populations and has been linked to increased risk of premature coronary artery disease. The Ser substitution leads to altered stability in the EGF-like and wire modules of the THBS-1 signature domain as assessed by differential scanning calorimetry carried out in 2 mm or 200 mum calcium. Studies of the melting profiles of the THBS-2 signature domain proteins with Asn or Ser at position 702 (homologous to 700 in THBS-1) revealed that the impact of the Ser allele is similar in both THBS-1 and THBS-2. Structure determination of the Ser(702) THBS-2 variant in 2 mm calcium showed that repeat 1C contains two bound calcium ions as in the crystal of the Asn(702) protein, including the ion that is coordinated by Asn(702), and is associated with changes in conformation of repeat 1C and the adjacent EGF-like modules. The Ser substitution leads to the decreased ability of soluble THBS-2 signature domain protein to bind 4B6.13, a conformation-sensitive monoclonal antibody that recognizes an epitope in repeat 1C. These results indicate that although THBS harboring the Ser allele binds a full complement of calcium ions, repeat 1C is altered, leading to destabilization of surrounding structures.

PubMed: 18499674
DOI: 10.1074/jbc.M800223200

実験手法

X-RAY DIFFRACTION (2.9 Å)