2RHO

Synthetic Gene Encoded Bacillus Subtilis FtsZ NCS Dimer with Bound GDP and GTP-gamma-S

Summary for 2RHO

• Entry DOI: 10.2210/pdb2rho/pdb
• Related: 2RHH 2RHJ 2RHL
• Descriptor: Cell Division Protein ftsZ, 5'-GUANOSINE-DIPHOSPHATE-MONOTHIOPHOSPHATE, GUANOSINE-5'-DIPHOSPHATE, ... (4 entities in total)
• Functional Keywords: cell division protein, tubulin homolog, gtp-binding, polymerization, gtpase, cell cycle, structural genomics, psi-2, protein structure initiative, accelerated technologies center for gene to 3d structure, atcg3d
• Biological source: Bacillus subtilis
• Cellular location: Cytoplasm : P17865
• Total number of polymer chains: 2
• Total formula weight: 69085.86

Authors

Lovell, S.,Halloran, Z.,Hjerrild, K.,Sheridan, D.,Burgin, A.,Stewart, L.,Accelerated Technologies Center for Gene to 3D Structure (ATCG3D) (deposition date: 2007-10-09, release date: 2008-10-21, Last modification date: 2024-02-21)

Primary citation

Raymond, A.,Lovell, S.,Lorimer, D.,Walchli, J.,Mixon, M.,Wallace, E.,Thompkins, K.,Archer, K.,Burgin, A.,Stewart, L.
Combined protein construct and synthetic gene engineering for heterologous protein expression and crystallization using Gene Composer.
BMC Biotechnol., 9:37-37, 2009

PubMed Abstract: With the goal of improving yield and success rates of heterologous protein production for structural studies we have developed the database and algorithm software package Gene Composer. This freely available electronic tool facilitates the information-rich design of protein constructs and their engineered synthetic gene sequences, as detailed in the accompanying manuscript.

PubMed: 19383143
DOI: 10.1186/1472-6750-9-37

Experimental method

X-RAY DIFFRACTION (2.45 Å)