2RHF

D. radiodurans RecQ HRDC domain 3

2RHF の概要

• エントリーDOI: 10.2210/pdb2rhf/pdb
• 分子名称: DNA helicase RecQ, PHOSPHATE ION (3 entities in total)
• 機能のキーワード: hrdc, recq, helicase, d. radiodurans, atp-binding, hydrolase, nucleotide-binding
• 由来する生物種: Deinococcus radiodurans
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 8581.35

構造登録者

Keck, J.L.,Killoran, M.P. (登録日: 2007-10-09, 公開日: 2008-04-22, 最終更新日: 2011-07-13)

主引用文献

Killoran, M.P.,Keck, J.L.
Structure and function of the regulatory C-terminal HRDC domain from Deinococcus radiodurans RecQ.
Nucleic Acids Res., 36:3139-3149, 2008

PubMed Abstract: RecQ helicases are critical for maintaining genome integrity in organisms ranging from bacteria to humans by participating in a complex network of DNA metabolic pathways. Their diverse cellular functions require specialization and coordination of multiple protein domains that integrate catalytic functions with DNA-protein and protein-protein interactions. The RecQ helicase from Deinococcus radiodurans (DrRecQ) is unusual among RecQ family members in that it has evolved to utilize three 'Helicase and RNaseD C-terminal' (HRDC) domains to regulate its activity. In this report, we describe the high-resolution structure of the C-terminal-most HRDC domain of DrRecQ. The structure reveals unusual electrostatic surface features that distinguish it from other HRDC domains. Mutation of individual residues in these regions affects the DNA binding affinity of DrRecQ and its ability to unwind a partial duplex DNA substrate. Taken together, the results suggest the unusual electrostatic surface features of the DrRecQ HRDC domain may be important for inter-domain interactions that regulate structure-specific DNA binding and help direct DrRecQ to specific recombination/repair sites.

PubMed: 18411208
DOI: 10.1093/nar/gkn143

実験手法

X-RAY DIFFRACTION (1.1 Å)