2RGS

FC-fragment of monoclonal antibody IGG2B from Mus musculus

2RGS の概要

• エントリーDOI: 10.2210/pdb2rgs/pdb
• 分子名称: Ig gamma-2B heavy chain, beta-D-galactopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-2)-alpha-D-mannopyranose-(1-6)-[2-acetamido-2-deoxy-beta-D-glucopyranose-(1-2)-alpha-D-mannopyranose-(1-3)]alpha-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-[alpha-L-fucopyranose-(1-6)]2-acetamido-2-deoxy-beta-D-glucopyranose (3 entities in total)
• 機能のキーワード: fc-fragment, immunoglobulin, glycosylation, immune system
• 由来する生物種: Mus musculus (mouse)
• 細胞内の位置: Isoform 1: Cell membrane; Single-pass membrane protein (Potential). Isoform 2: Secreted: P01867
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 52312.55

構造登録者

Kolenko, P.,Duskova, J.,Skalova, T.,Dohnalek, J.,Hasek, J. (登録日: 2007-10-05, 公開日: 2008-10-07, 最終更新日: 2024-11-13)

主引用文献

Kolenko, P.,Dohnalek, J.,Duskova, J.,Skalova, T.,Collard, R.,Hasek, J.
New insights into intra- and intermolecular interactions of immunoglobulins: crystal structure of mouse IgG2b-Fc at 2.1-A resolution
Immunology, 126:378-385, 2008

PubMed Abstract: The structure of the Fc fragment of monoclonal antibody IgG2b from hybridom M75 of Mus musculus has been determined by single crystal X-ray diffraction. This is the first report of the structure of the murine immunoglobulin isotype IgG2b. The structure refined at 2.1 A resolution provides more detailed structural information about native oligosaccharides than was previously available. High-quality Fourier maps provide a clear identification of alpha-l-fucose with partial occupancy in the first branch of the antennary oligosaccharides. A unique Fc:Fc interaction was observed at the C(H)2-C(H)3 interface.

PubMed: 18783468
DOI: 10.1111/j.1365-2567.2008.02904.x

実験手法

X-RAY DIFFRACTION (2.13 Å)