2RFK

Substrate RNA Positioning in the Archaeal H/ACA Ribonucleoprotein Complex

2RFK の概要

• エントリーDOI: 10.2210/pdb2rfk/pdb
• 分子名称: guide RNA 1, guide RNA 2, target RNA, ... (7 entities in total)
• 機能のキーワード: protein-rna complex, archaeal h-aca ribonucleoprotein complex, isomerase, trna processing, ribosome biogenesis, rrna processing, isomerase-rna complex, structural genomics, southeast collaboratory for structural genomics, secsg, psi-2, protein structure initiative, isomerase/rna
• 由来する生物種: Pyrococcus furiosus; 詳細
• タンパク質・核酸の鎖数: 6
• 化学式量合計: 72358.01

構造登録者

Liang, B.,Xue, S.,Terns, R.M.,Terns, M.P.,Li, H.,Southeast Collaboratory for Structural Genomics (SECSG) (登録日: 2007-09-30, 公開日: 2008-02-05, 最終更新日: 2024-10-30)

主引用文献

Liang, B.,Xue, S.,Terns, R.M.,Terns, M.P.,Li, H.
Substrate RNA positioning in the archaeal H/ACA ribonucleoprotein complex.
Nat.Struct.Mol.Biol., 14:1189-1195, 2007

PubMed Abstract: The most complex RNA pseudouridylases are H/ACA ribonucleoprotein particles, which use a guide RNA for substrate capture and four proteins (Cbf5, Nop10, Gar1 and L7Ae/NHP2) for substrate modification. Here we report the three-dimensional structure of a catalytically deficient archaeal enzyme complex (including the guide RNA and three of the four essential proteins) bound to a substrate RNA. Extensive interactions of Cbf5 with one guide-substrate helix and a guide RNA stem shape the forked guide–substrate RNA complex structure and position the substrate in proximity of the Cbf5 catalytic center. Our structural and complementary fluorescence analyses also indicate that precise placement of the target uridine at the active site requires a conformation of the guide–substrate RNA duplex that is brought about by the previously identified concurrent interaction of the guide RNA with L7Ae and a composite Cbf5-Nop10 surface, and further identify a residue that is critical in this process.

PubMed: 18059286
DOI: 10.1038/nsmb1336

実験手法

X-RAY DIFFRACTION (2.87 Å)