2REB

THE STRUCTURE OF THE E. COLI RECA PROTEIN MONOMER AND POLYMER

2REB の概要

• エントリーDOI: 10.2210/pdb2reb/pdb
• 分子名称: REC A (2 entities in total)
• 機能のキーワード: self-cleavage stimulation, homologous recombination, dna binding protein
• 由来する生物種: Escherichia coli
• 細胞内の位置: Cytoplasm: P0A7G6
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 37885.09

構造登録者

Story, R.M.,Steitz, T.A. (登録日: 1992-03-06, 公開日: 1993-10-31, 最終更新日: 2024-02-21)

主引用文献

Story, R.M.,Weber, I.T.,Steitz, T.A.
The structure of the E. coli recA protein monomer and polymer.
Nature, 355:318-325, 1992

PubMed Abstract: The crystal structure of the recA protein from Escherichia coli at 2.3-A resolution reveals a major domain that binds ADP and probably single- and double-stranded DNA. Two smaller subdomains at the N and C termini protrude from the protein and respectively stabilize a 6(1) helical polymer of protein subunits and interpolymer bundles. This polymer structure closely resembles that of recA/DNA filaments determined by electron microscopy. Mutations in recA protein that enhance coprotease, DNA-binding and/or strand-exchange activity can be explained if the interpolymer interactions in the crystal reflect a regulatory mechanism in vivo.

PubMed: 1731246
DOI: 10.1038/355318a0

実験手法

X-RAY DIFFRACTION (2.3 Å)