2RC8

Crystal structure of the NR3A ligand binding core complex with D-serine at 1.45 Angstrom resolution

2RC8 の概要

• エントリーDOI: 10.2210/pdb2rc8/pdb
• 関連するPDBエントリー: 1PB7 1PB8 2RC7 2RC9 2RCA 2RCB
• 分子名称: Glutamate [NMDA] receptor subunit 3A, CHLORIDE ION, D-SERINE, ... (5 entities in total)
• 機能のキーワード: membrane protein, cell junction, glycoprotein, ion transport, ionic channel, magnesium, postsynaptic cell membrane, receptor, synapse, transmembrane, transport
• 由来する生物種: Rattus norvegicus (Rat); 詳細
• 細胞内の位置: Cell membrane; Multi-pass membrane protein: Q9R1M7
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 66465.43

構造登録者

Yao, Y.,Mayer, M.L. (登録日: 2007-09-19, 公開日: 2008-08-05, 最終更新日: 2024-10-30)

主引用文献

Yao, Y.,Harrison, C.B.,Freddolino, P.L.,Schulten, K.,Mayer, M.L.
Molecular mechanism of ligand recognition by NR3 subtype glutamate receptors.
Embo J., 27:2158-2170, 2008

PubMed Abstract: NR3 subtype glutamate receptors have a unique developmental expression profile, but are the least well-characterized members of the NMDA receptor gene family, which have key roles in synaptic plasticity and brain development. Using ligand binding assays, crystallographic analysis, and all atom MD simulations, we investigate mechanisms underlying the binding by NR3A and NR3B of glycine and D-serine, which are candidate neurotransmitters for NMDA receptors containing NR3 subunits. The ligand binding domains of both NR3 subunits adopt a similar extent of domain closure as found in the corresponding NR1 complexes, but have a unique loop 1 structure distinct from that in all other glutamate receptor ion channels. Within their ligand binding pockets, NR3A and NR3B have strikingly different hydrogen bonding networks and solvent structures from those found in NR1, and fail to undergo a conformational rearrangement observed in NR1 upon binding the partial agonist ACPC. MD simulations revealed numerous interdomain contacts, which stabilize the agonist-bound closed-cleft conformation, and a novel twisting motion for the loop 1 helix that is unique in NR3 subunits.

PubMed: 18636091
DOI: 10.1038/emboj.2008.140

実験手法

X-RAY DIFFRACTION (1.45 Å)