2R5Y

Structure of Scr/Exd complex bound to a consensus Hox-Exd site

2R5Y の概要

• エントリーDOI: 10.2210/pdb2r5y/pdb
• 関連するPDBエントリー: 1B8I 2R5Z
• 分子名称: DNA (5'-D(*DAP*DCP*DTP*DCP*DTP*DAP*DTP*DGP*DAP*DTP*DTP*DTP*DAP*DTP*DGP*DGP*DGP*DCP*DTP*DG)-3'), DNA (5'-D(*DTP*DCP*DAP*DGP*DCP*DCP*DCP*DAP*DTP*DAP*DAP*DAP*DTP*DCP*DAP*DTP*DAP*DGP*DAP*DG)-3'), Homeotic protein Sex combs reduced, ... (5 entities in total)
• 機能のキーワード: homeodomain, homeotic proteins, specificity, developmental protein, dna-binding, homeobox, nucleus, transcription, transcription regulation, transcription-dna complex, transcription/dna
• 由来する生物種: Drosophila melanogaster (fruit fly); 詳細
• 細胞内の位置: Nucleus: P09077 P40427
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 30787.25

構造登録者

Aggarwal, A.K.,Passner, J.M.,Jain, R. (登録日: 2007-09-04, 公開日: 2008-02-05, 最終更新日: 2023-08-30)

主引用文献

Joshi, R.,Passner, J.M.,Rohs, R.,Jain, R.,Sosinsky, A.,Crickmore, M.A.,Jacob, V.,Aggarwal, A.K.,Honig, B.,Mann, R.S.
Functional specificity of a Hox protein mediated by the recognition of minor groove structure
Cell(Cambridge,Mass.), 131:530-543, 2007

PubMed Abstract: The recognition of specific DNA-binding sites by transcription factors is a critical yet poorly understood step in the control of gene expression. Members of the Hox family of transcription factors bind DNA by making nearly identical major groove contacts via the recognition helices of their homeodomains. In vivo specificity, however, often depends on extended and unstructured regions that link Hox homeodomains to a DNA-bound cofactor, Extradenticle (Exd). Using a combination of structure determination, computational analysis, and in vitro and in vivo assays, we show that Hox proteins recognize specific Hox-Exd binding sites via residues located in these extended regions that insert into the minor groove but only when presented with the correct DNA sequence. Our results suggest that these residues, which are conserved in a paralog-specific manner, confer specificity by recognizing a sequence-dependent DNA structure instead of directly reading a specific DNA sequence.

PubMed: 17981120
DOI: 10.1016/j.cell.2007.09.024

実験手法

X-RAY DIFFRACTION (2.6 Å)