2R2Y

Crystal structure of the proteasomal Rpn13 PRU-domain

2R2Y の概要

• エントリーDOI: 10.2210/pdb2r2y/pdb
• 関連するPDBエントリー: 2Z59
• 分子名称: Protein ADRM1 (2 entities in total)
• 機能のキーワード: proteasome, ubiquitin, ph-domain, 19s regulator, receptor, ubiquitin-proteasome-degradation pathway, receptor domain for ubiquitin and proteasome binding, protein binding
• 由来する生物種: Mus musculus (house mouse)
• 細胞内の位置: Cytoplasm: Q9JKV1
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 16855.12

構造登録者

Groll, M.,Walters, K.,Dikic, I.,Finley, D. (登録日: 2007-08-28, 公開日: 2008-05-13, 最終更新日: 2024-02-21)

主引用文献

Schreiner, P.,Chen, X.,Husnjak, K.,Randles, L.,Zhang, N.,Elsasser, S.,Finley, D.,Dikic, I.,Walters, K.J.,Groll, M.
Ubiquitin docking at the proteasome through a novel pleckstrin-homology domain interaction.
Nature, 453:548-552, 2008

PubMed Abstract: Targeted protein degradation is largely performed by the ubiquitin-proteasome pathway, in which substrate proteins are marked by covalently attached ubiquitin chains that mediate recognition by the proteasome. It is currently unclear how the proteasome recognizes its substrates, as the only established ubiquitin receptor intrinsic to the proteasome is Rpn10/S5a (ref. 1), which is not essential for ubiquitin-mediated protein degradation in budding yeast. In the accompanying manuscript we report that Rpn13 (refs 3-7), a component of the nine-subunit proteasome base, functions as a ubiquitin receptor, complementing its known role in docking de-ubiquitinating enzyme Uch37/UCHL5 (refs 4-6) to the proteasome. Here we merge crystallography and NMR data to describe the ubiquitin-binding mechanism of Rpn13. We determine the structure of Rpn13 alone and complexed with ubiquitin. The co-complex reveals a novel ubiquitin-binding mode in which loops rather than secondary structural elements are used to capture ubiquitin. Further support for the role of Rpn13 as a proteasomal ubiquitin receptor is demonstrated by its ability to bind ubiquitin and proteasome subunit Rpn2/S1 simultaneously. Finally, we provide a model structure of Rpn13 complexed to diubiquitin, which provides insights into how Rpn13 as a ubiquitin receptor is coupled to substrate deubiquitination by Uch37.

PubMed: 18497827
DOI: 10.1038/nature06924

実験手法

X-RAY DIFFRACTION (1.7 Å)