2R2F

RIBONUCLEOTIDE REDUCTASE R2F PROTEIN FROM SALMONELLA TYPHIMURIUM (OXIDIZED)

2R2F の概要

• エントリーDOI: 10.2210/pdb2r2f/pdb
• 分子名称: PROTEIN (RIBONUCLEOSIDE-DIPHOSPHATE REDUCTASE SMALL CHAIN), MU-OXO-DIIRON (3 entities in total)
• 機能のキーワード: reductase, nucleotide metabolism, oxidoreductase
• 由来する生物種: Salmonella typhimurium
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 72781.35

構造登録者

Eklund, H.,Eriksson, M. (登録日: 1998-08-24, 公開日: 1999-01-13, 最終更新日: 2023-12-27)

主引用文献

Eriksson, M.,Jordan, A.,Eklund, H.
Structure of Salmonella typhimurium nrdF ribonucleotide reductase in its oxidized and reduced forms.
Biochemistry, 37:13359-13369, 1998

PubMed Abstract: The first class Ib ribonucleotide reductase R2 structure, from Salmonella typhimurium, has been determined at 2.0 A resolution. The overall structure is similar to the Escherichia coli class Ia enzyme despite only 23% sequence identity. The most spectacular difference is the absence of the pleated sheet and adjacent parts present in the E. coli R2 structure; the heart-shaped structure loses its tip. From sequence comparisons, it appears that this feature is shared with all other class Ib enzymes and, in this respect, is more like the mammalian class Ia enzymes. Both the oxidized and reduced iron forms have been investigated. In the ferric iron center, both iron ions are octahedrally coordinated and bridged by one carboxylate and one oxide ion. The ferrous form has lost the bridging oxide ion but is bridged by two carboxylates. Accompanying the change in redox state, helix E changes its conformation from one covering the metal center in the oxidized form to a more open reduced form. A narrow channel is opened which may permit easier access of oxygen to the ferrous iron site and to efficiently generate the tyrosyl radical.

PubMed: 9748343
DOI: 10.1021/bi981380s

実験手法

X-RAY DIFFRACTION (2.25 Å)