2R1B

Crystal Structure of rat neurexin 1beta with a splice insert at SS#4

Summary for 2R1B

• Entry DOI: 10.2210/pdb2r1b/pdb
• Related: 2R16 2R1D
• Descriptor: Neurexin-1-beta, CALCIUM ION (3 entities in total)
• Functional Keywords: beta-sandwich, cell adhesion, splicing
• Biological source: Rattus norvegicus (Norway rat)
• Total number of polymer chains: 2
• Total formula weight: 48069.95

Authors

Rudenko, G. (deposition date: 2007-08-22, release date: 2008-03-25, Last modification date: 2023-08-30)

Primary citation

Shen, K.C.,Kuczynska, D.A.,Wu, I.J.,Murray, B.H.,Sheckler, L.R.,Rudenko, G.
Regulation of Neurexin 1beta Tertiary Structure and Ligand Binding through Alternative Splicing
Structure, 16:422-431, 2008

PubMed Abstract: Neurexins and neuroligins play an essential role in synapse function, and their alterations are linked to autistic spectrum disorder. Interactions between neurexins and neuroligins regulate inhibitory and excitatory synaptogenesis in vitro through a "splice-insert signaling code." In particular, neurexin 1beta carrying an alternative splice insert at site SS#4 interacts with neuroligin 2 (found predominantly at inhibitory synapses) but much less so with other neuroligins (those carrying an insert at site B and prevalent at excitatory synapses). The structure of neurexin 1beta+SS#4 reveals dramatic rearrangements to the "hypervariable surface," the binding site for neuroligins. The splice insert protrudes as a long helix into space, triggers conversion of loop beta10-beta11 into a helix rearranging the binding site for neuroligins, and rearranges the Ca(2+)-binding site required for ligand binding, increasing its affinity. Our structures reveal the mechanism by which neurexin 1beta isoforms acquire neuroligin splice isoform selectivity.

PubMed: 18334217
DOI: 10.1016/j.str.2008.01.005

Experimental method

X-RAY DIFFRACTION (1.72 Å)