2R1B

Crystal Structure of rat neurexin 1beta with a splice insert at SS#4

2R1B の概要

• エントリーDOI: 10.2210/pdb2r1b/pdb
• 関連するPDBエントリー: 2R16 2R1D
• 分子名称: Neurexin-1-beta, CALCIUM ION (3 entities in total)
• 機能のキーワード: beta-sandwich, cell adhesion, splicing
• 由来する生物種: Rattus norvegicus (Norway rat)
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 48069.95

構造登録者

Rudenko, G. (登録日: 2007-08-22, 公開日: 2008-03-25, 最終更新日: 2023-08-30)

主引用文献

Shen, K.C.,Kuczynska, D.A.,Wu, I.J.,Murray, B.H.,Sheckler, L.R.,Rudenko, G.
Regulation of Neurexin 1beta Tertiary Structure and Ligand Binding through Alternative Splicing
Structure, 16:422-431, 2008

PubMed Abstract: Neurexins and neuroligins play an essential role in synapse function, and their alterations are linked to autistic spectrum disorder. Interactions between neurexins and neuroligins regulate inhibitory and excitatory synaptogenesis in vitro through a "splice-insert signaling code." In particular, neurexin 1beta carrying an alternative splice insert at site SS#4 interacts with neuroligin 2 (found predominantly at inhibitory synapses) but much less so with other neuroligins (those carrying an insert at site B and prevalent at excitatory synapses). The structure of neurexin 1beta+SS#4 reveals dramatic rearrangements to the "hypervariable surface," the binding site for neuroligins. The splice insert protrudes as a long helix into space, triggers conversion of loop beta10-beta11 into a helix rearranging the binding site for neuroligins, and rearranges the Ca(2+)-binding site required for ligand binding, increasing its affinity. Our structures reveal the mechanism by which neurexin 1beta isoforms acquire neuroligin splice isoform selectivity.

PubMed: 18334217
DOI: 10.1016/j.str.2008.01.005

実験手法

X-RAY DIFFRACTION (1.72 Å)