2QW1

Glucose/galactose binding protein bound to 3-O-methyl D-glucose

2QW1 の概要

• エントリーDOI: 10.2210/pdb2qw1/pdb
• 関連するPDBエントリー: 2FVY 2FW0 2GBP
• 分子名称: D-galactose-binding periplasmic protein, 3-O-methyl-beta-D-glucopyranose, CALCIUM ION, ... (5 entities in total)
• 機能のキーワード: periplasmic binding protein, antagonist, chemotaxis, transport, ggbp, 3-o-methyl glucose, sugar transport, transport protein
• 由来する生物種: Escherichia coli
• 細胞内の位置: Periplasm: P0AEE5
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 33687.87

構造登録者

Borrok, M.J.,Kiessling, L.L.,Forest, K.T. (登録日: 2007-08-09, 公開日: 2008-08-05, 最終更新日: 2023-08-30)

主引用文献

Borrok, M.J.,Zhu, Y.,Forest, K.T.,Kiessling, L.L.
Structure-based design of a periplasmic binding protein antagonist that prevents domain closure.
Acs Chem.Biol., 4:447-456, 2009

PubMed Abstract: Many receptors undergo ligand-induced conformational changes to initiate signal transduction. Periplasmic binding proteins (PBPs) are bacterial receptors that exhibit dramatic conformational changes upon ligand binding. These proteins mediate a wide variety of fundamental processes including transport, chemotaxis, and quorum sensing. Despite the importance of these receptors, no PBP antagonists have been identified and characterized. In this study, we identify 3-O-methyl-d-glucose as an antagonist of glucose/galactose-binding protein and demonstrate that it inhibits glucose chemotaxis in E. coli. Using small-angle X-ray scattering and X-ray crystallography, we show that this antagonist acts as a wedge. It prevents the large-scale domain closure that gives rise to the active signaling state. Guided by these results and the structures of open and closed glucose/galactose-binding protein, we designed and synthesized an antagonist composed of two linked glucose residues. These findings provide a blueprint for the design of new bacterial PBP inhibitors. Given the key role of PBPs in microbial physiology, we anticipate that PBP antagonists will have widespread uses as probes and antimicrobial agents.

PubMed: 19348466
DOI: 10.1021/cb900021q

実験手法

X-RAY DIFFRACTION (1.7 Å)