2QRO

Human Deoxycytidine kinase dAMP, UDP, Mg ion product complex

Summary for 2QRO

• Entry DOI: 10.2210/pdb2qro/pdb
• Related: 2QRN
• Descriptor: Deoxycytidine kinase, MAGNESIUM ION, URIDINE-5'-DIPHOSPHATE, ... (4 entities in total)
• Functional Keywords: deoxycytidine kinase, deoxyadenosine monophosphate, uridine diphosphate, atp-binding, nucleotide-binding, nucleus, phosphorylation, transferase
• Biological source: Homo sapiens (human)
• Cellular location: Nucleus: P27707
• Total number of polymer chains: 4
• Total formula weight: 133934.15

Authors

Ealick, S.E.,Soriano, E.V. (deposition date: 2007-07-28, release date: 2007-12-11, Last modification date: 2023-08-30)

Primary citation

Soriano, E.V.,Clark, V.C.,Ealick, S.E.
Structures of human deoxycytidine kinase product complexes.
Acta Crystallogr.,Sect.D, 63:1201-1207, 2007

PubMed Abstract: Human deoxycytidine kinase (dCK) is involved in the nucleotide-biosynthesis salvage pathway and has also been shown to phosphorylate several antitumor and antiviral prodrugs. The structures of dCK alone and the dead-end complex of dCK with substrate nucleoside and product ADP or UDP have previously been reported; however, there is currently no structure available for a substrate or product complex. Here, the structures of dCK complexes with the products dCMP, UDP and Mg2+ ion, and with dAMP, UDP and Mg2+ ion are reported. Structural comparisons show that the product complexes with UDP and a dead-end complex with substrate and UDP have similar active-site conformations.

PubMed: 18084067
DOI: 10.1107/S0907444907048834

Experimental method

X-RAY DIFFRACTION (3.45 Å)