2QOP

Crystal structure of the transcriptional regulator AcrR from Escherichia coli

2QOP の概要

• エントリーDOI: 10.2210/pdb2qop/pdb
• 分子名称: HTH-type transcriptional regulator acrR (2 entities in total)
• 機能のキーワード: acrb regulator, dna-binding, repressor, transcription, transcription regulation
• 由来する生物種: Escherichia coli K12
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 24797.66

構造登録者

Li, M.,Gu, R.,Su, C.-C.,McDermott, G.,Yu, E.W. (登録日: 2007-07-20, 公開日: 2008-02-26, 最終更新日: 2024-02-21)

主引用文献

Li, M.,Gu, R.,Su, C.C.,Routh, M.D.,Harris, K.C.,Jewell, E.S.,McDermott, G.,Yu, E.W.
Crystal structure of the transcriptional regulator AcrR from Escherichia coli.
J.Mol.Biol., 374:591-603, 2007

PubMed Abstract: The AcrAB multidrug efflux pump, which belongs to the resistance nodulation division (RND) family, recognizes and extrudes a wide range of antibiotics and chemotherapeutic agents and causes the intrinsic antibiotic resistance in Escherichia coli. The expression of AcrAB is controlled by the transcriptional regulator AcrR, whose open reading frame is located 141 bp upstream of the acrAB operon. To understand the structural basis of AcrR regulation, we have determined the crystal structure of AcrR to 2.55-A resolution, revealing a dimeric two-domain molecule with an entirely helical architecture similar to members of the TetR family of transcriptional regulators. Each monomer of AcrR forms a multientrance pocket of 350 A(3) in the ligand-binding domain. The ligand-binding pocket is surrounded with mostly hydrophobic residues. In addition, a completely buried negatively charged glutamate, expected to be critical for drug binding, is located at the center of the binding pocket. The crystal structure provides novel insight into the mechanisms of ligand binding and AcrR regulation.

PubMed: 17950313
DOI: 10.1016/j.jmb.2007.09.064

実験手法

X-RAY DIFFRACTION (2.55 Å)