2QNO

Crystal Structure of the Mutant E55Q of the Cellulase CEL48F in Complex with a Thio-Oligosaccharide

2QNO の概要

• エントリーDOI: 10.2210/pdb2qno/pdb
• 関連するPDBエントリー: 1g9g 1g9j
• 分子名称: Endoglucanase F, 4-thio-beta-D-glucopyranose-(1-4)-beta-D-glucopyranose-(1-4)-4-thio-beta-D-glucopyranose-(1-4)-beta-D-glucopyranose-(1-4)-4-thio-beta-D-glucopyranose-(1-4)-beta-D-glucopyranose-(1-4)-4-thio-beta-D-glucopyranose-(1-4)-beta-D-glucopyranose-(1-4)-4-thio-beta-D-glucopyranose, CALCIUM ION, ... (4 entities in total)
• 機能のキーワード: cellulase, thio-oligosaccharide complex, inactive mutant, gh family 48, alpha-alpha-six barrel, carbohydrate metabolism, cellulose degradation, glycosidase, hydrolase, polysaccharide degradation
• 由来する生物種: Clostridium cellulolyticum
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 72466.58

構造登録者

Parsiegla, G.,Haser, R. (登録日: 2007-07-19, 公開日: 2008-01-15, 最終更新日: 2023-08-30)

• 主引用文献: Parsiegla, G.,Reverbel, C.,Tardif, C.,Driguez, H.,Haser, R.; Structures of mutants of cellulase Cel48F of Clostridium cellulolyticum in complex with long hemithiocellooligosaccharides give rise to a new view of the substrate pathway during processive action; J.Mol.Biol., 375:499-510, 2008; PubMed: 18035374; DOI: 10.1016/j.jmb.2007.10.039

実験手法

X-RAY DIFFRACTION (2 Å)