2QH4

Solution structure of the U85 C/D-H/ACA scaRNA 5' terminal hairpin loop

2QH4 の概要

• エントリーDOI: 10.2210/pdb2qh4/pdb
• NMR情報: BMRB: 7405
• 分子名称: Human U85 C/D-H/ACA scaRNA (1 entity in total)
• 機能のキーワード: loop, sheared ga pair, rna
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 5788.49

構造登録者

Feigon, J.,Theimer, C.A.,Chim, N.,Breece, K.E. (登録日: 2007-06-29, 公開日: 2007-09-25, 最終更新日: 2024-05-22)

主引用文献

Theimer, C.A.,Jady, B.E.,Chim, N.,Richard, P.,Breece, K.E.,Kiss, T.,Feigon, J.
Structural and functional characterization of human telomerase RNA processing and cajal body localization signals.
Mol.Cell, 27:869-881, 2007

PubMed Abstract: The RNA component of human telomerase (hTR) includes H/ACA and CR7 domains required for 3' end processing, localization, and accumulation. The terminal loop of the CR7 domain contains the CAB box (ugAG) required for targeting of scaRNAs to Cajal bodies (CB) and an uncharacterized sequence required for accumulation and processing. To dissect out the contributions of the CR7 stem loop to hTR processing and localization, we solved the solution structures of the 3' terminal stem loops of hTR CR7 and U64 H/ACA snoRNA, and the 5' terminal stem loop of U85 C/D-H/ACA scaRNA. These structures, together with analysis of localization, processing, and accumulation of hTRs containing nucleotide substitutions in the CR7 domain, identified the sequence and structural requirements of the hTR processing and CB localization signals and showed that these signals are functionally independent. Further, 3' end processing was found to be a prerequisite for translocation of hTR to CBs.

PubMed: 17889661
DOI: 10.1016/j.molcel.2007.07.017

実験手法

SOLUTION NMR